Electron tomography of large, multicomponent biological structures

被引：89

作者：

Perkins, GA ^{[1
]}

Renken, CW

Song, JY

Frey, TG

Young, SJ

Lamont, S

Martone, ME

Lindsey, S

Ellisman, MH

机构：

[1] Univ Calif San Diego, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA

[2] San Diego State Univ, Dept Biol, San Diego, CA 92182 USA

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 1997年 / 120卷 / 03期

关键词：

D O I：

10.1006/jsbi.1997.3920

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron tomography is an extremely useful method for deriving three-dimensional structure from electron microscope images. The application of this technique to the reconstruction of large, complex structures such as mitochondria is described in conjunction with several tools for segmentation, measurement, classification, and visualization. In addition, the use of massively parallel computers to perform the tomographic reconstruction efficiently using R-weighted backprojection or iterative techniques is described. (C) 1998 Academic Press.

引用

页码：219 / 227

页数：9

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