Cloning and expression study of a putative high-affinity nitrate transporter gene from Dunaliella salina

被引：25

作者：

He, QH ^{[1
]}

Qiao, DR ^{[1
]}

Zhang, QL ^{[1
]}

Li, Y ^{[1
]}

Xu, H ^{[1
]}

Wei, L ^{[1
]}

Gu, Y ^{[1
]}

Cao, Y ^{[1
]}

机构：

[1] Sichuan Univ, Key Lab Dept Educ Biol Resource & Ecol Environm, Chengdu 610064, Sichuan Provinc, Peoples R China

来源：

JOURNAL OF APPLIED PHYCOLOGY | 2004年 / 16卷 / 05期

关键词：

Dunaliella salina; nitrate transporter gene; nitrate; salt stress;

D O I：

10.1023/B:JAPH.0000047950.76549.ce

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A nitrate transporter gene, named DsNRT2.1 (GeneBank accession number AY621079), from Dunaliella salina has been cloned by screening a cDNA library, which was constructed with mRNAs from D. salina after 60 min treatment with 5 mM nitrate, with a 342 bp NRT2 cDNA fragment from D. salina as a probe. DsNRT2.1 exhibits sequence similarity to those known nitrate transporters of the NRT2 family. A hydrophobicity blot indicated that DsNRT2.1 belongs to the major facilitator superfamily (MFS). Northern analysis showed that an mRNA species of 1.9 kb can be rapidly induced by NO3-, but not by NH4+. Northern analysis also showed that NaCl could significantly increase the expression of DsNRT2.1.

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页码：395 / 400

页数：6

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