Activation of β-catenin in the stem cell region of crypts during growth of the small intestine in infant rats

被引：12

作者：

Camac, Kimberley S.

Thompson, Fiona M.

Cummins, Adrian G. ^{[1
]}

机构：

[1] Queen Elizabeth Hosp, Dept Gastroenterol & Hepatol, Woodville, SA 5011, Australia

[2] Univ S Australia, Adelaide, SA 5001, Australia

[3] Univ Adelaide, Adelaide, SA, Australia

来源：

DIGESTIVE DISEASES AND SCIENCES | 2007年 / 52卷 / 05期

基金：

英国医学研究理事会;

关键词：

beta catenin; growth factor; intestinal growth; intestinal stem cell;

D O I：

10.1007/s10620-006-9200-7

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The Wnt/beta-catenin pathway has been proposed as promoting intestinal stem cell division. Wnt ligands activate cytoplasmic beta-catenin and increase nuclear translocation of beta-catenin that binds to the Tcf-4 transcription factor. The aim of this study was to investigate beta-catenin expression in the stem cell region of crypts during intestinal growth in rats. Litters of DAxPVG/c rats were humanely killed at 7, 14, 21, 35, and 72 days of life. beta-Catenin and Tcf-4 were quantified by immunoperoxidase staining and image analysis with cumulative signal analysis. Cytoplasmic and nuclear expression of beta-catenin peaked nearly 2-fold at day 14 (versus day 7) of life in the stem cell region of intestinal crypts. Tcf-4 nuclear expression peaked earlier at 7 days and was lower thereafter with age. We conclude that the Wnt/beta-catenin pathway is activated in the stem cell region of intestinal crypts during growth of the small intestine.

引用

页码：1242 / 1246

页数：5

共 28 条

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