Crystallization and preliminary X-ray crystallographic studies of chorismate synthase from Helicobacter pylori

被引：4

作者：

Ahn, HJ ^{[1
]}

Yang, JK ^{[1
]}

Lee, BI ^{[1
]}

Yoon, HJ ^{[1
]}

Kim, HW ^{[1
]}

Suh, SW ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Chem & Mol Engn, Struct Proteom Lab, Seoul 151742, South Korea

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2003年 / 59卷

关键词：

D O I：

10.1107/S090744490300009X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chorismate synthase (EC 4.6.1.4) catalyzes the transformation of 5-enolpyruvylshikimate 3-phosphate to chorismate in the last step of the shikimate pathway. Chorismate synthase from Helicobacter pylori fused with an eight-residue C-terminal tag was overexpressed in soluble form in Escherichia coli. It was crystallized at 296 K using polyethylene glycol 400 as a precipitant. A set of X-ray diffraction data was collected to 2.5 Angstrom resolution using synchrotron radiation. The crystals belong to the tetragonal space group I4, with unit-cell parameters a = b = 145.79, c = 130.98 Angstrom. The asymmetric unit contains a tetramer, giving a crystal volume per protein mass (V-M) of 2.13 Angstrom(3) Da(-1) and a solvent content of 42.3%.

引用

页码：569 / 571

页数：3

共 20 条

[1] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].

BAILEY, S .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763

[2] Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].

Brunger, AT ;

Adams, PD ;

Clore, GM ;

DeLano, WL ;

Gros, P ;

Grosse-Kunstleve, RW ;

Jiang, JS ;

Kuszewski, J ;

Nilges, M ;

Pannu, NS ;

Read, RJ ;

Rice, LM ;

Simonson, T ;

Warren, GL .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921

[3] STEREOCHEMISTRY OF CHORISMIC ACID BIOSYNTHESIS [J].

HILL, RK ;

NEWKOME, GR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1969, 91 (21) :5893-&

[4] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[5] A unique reaction in a common pathway: mechanism and function of chorismate synthase in the shikimate pathway [J].

Macheroux, P ;

Schmid, J ;

Amrhein, N ;

Schaller, A .

PLANTA, 1999, 207 (03) :325-334

[6] Evidence for a major structural change in Escherichia coli chorismate synthase induced by flavin and substrate binding [J].

Macheroux, P ;

Schönbrunn, E ;

Svergun, DI ;

Volkov, VV ;

Koch, MHJ ;

Bornemann, S ;

Thorneley, RNF .

BIOCHEMICAL JOURNAL, 1998, 335 :319-327

[7] SOLVENT CONTENT OF PROTEIN CRYSTALS [J].

MATTHEWS, BW .

JOURNAL OF MOLECULAR BIOLOGY, 1968, 33 (02) :491-+

[8] Ubiquinone biosynthesis in microorganisms [J].

Meganathan, R .

FEMS MICROBIOLOGY LETTERS, 2001, 203 (02) :131-139

[9] Over-production of proteins in Escherichia coli: Mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels [J].

Miroux, B ;

Walker, JE .

JOURNAL OF MOLECULAR BIOLOGY, 1996, 260 (03) :289-298

[10]

MORELL H, 1967, J BIOL CHEM, V242, P82

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