Cell wall properties of transgenic tobacco plants that express a yeast derived acid invertase in their vacuole

被引：5

作者：

HoffmannBenning, S

Ehwald, R

Willmitzer, L

Fisahn, J

机构：

[1] Institut für Genbiologische Forschung, 14193 Berlin

[2] Humbold Universität, Sektion Biologie, 1040 Berlin

[3] Max Planck Institut für molekulare Pflanzenphysiologie, Karl, 14476 Golm

来源：

PECTINS AND PECTINASES | 1996年 / 14卷

关键词：

D O I：

10.1016/S0921-0423(96)80301-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Transgenic tobacco plants that express a yeast derived acid invertase in their vacuole were used to analyse the effects of a modification in carbon allocation on cell wall properties and turgor pressure. Significant increases in turgor pressure, free and total acid content were detected in the transgenic lines. Cell wall neutral sugars were examined by gas chromatography and as a result the arabinose concentration was found to be significantly increased in leaf 6 of the transgenic plants. In contrast, fucose was reduced. However, the size exclusion limit of the pectin network was unaffected by the genetic manipulation.

引用

页码：667 / 671

页数：5

共 10 条

[1]

Heineke D., Wildenberger K., Sonnewald U., Willmitzer L., Held H.W., Planta, 194, pp. 29-33, (1994)

[2]

Sonnewald U., Brauer M., van Schaewen A., Stitt M., Willmitzer L., Plant J., 1, (1991)

[3]

Hoffmann-Benning S., Willmitzer L., Fisahn J., Plant Physiol., (1996)

[4]

Selvendran R.R., Ryden P., Methods in Plant Biochem, (1990)

[5]

Ehwald R., Woehlecke H., Titel C., Phytochem., 31, (1992)

[6]

Ehwald R., Heese P., Klein U., J. Chromatography, 542, (1991)

[7]

Husken D., Steudle E., Zimmermann U., Pant Physiol., 61, (1978)

[8]

Lockhart J.A., J. Theor. Biol., 8, (1965)

[9]

McQuenn-Mason S.J., J. Exp. Bot., 292, (1965)

[10]

Cosgrove D.J., Suvonick-Dunford S.A., Plant Physiol., 89, (1989)

← 1 →