Expression of adrenomedullin mRNA in the human brain and pituitary

被引：57

作者：

Takahashi, K

Satoh, F

Sone, M

Murakami, O

Sasano, H

Mouri, T

Shibahara, S

机构：

[1] TOHOKU UNIV, SCH MED, DEPT INTERNAL MED 2, SENDAI, MIYAGI 98077, JAPAN

[2] TOHOKU UNIV, SCH MED, DEPT PATHOL 2, SENDAI, MIYAGI 98077, JAPAN

[3] NATL IWATE HOSP, ICHINOSEKI, IWATE 021, JAPAN

来源：

PEPTIDES | 1997年 / 18卷 / 07期

关键词：

adrenomedullin; radioimmunoassay; RNA; Northern blot; pituitary; hypothalamus; brain;

D O I：

10.1016/S0196-9781(97)00038-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recently reported the presence of immunoreactive (IR) adrenomedullin (ADM) in the human brain. In the present study, the expression of ADM mRNA was studied by Northern blot analysis in the human brain and pituitary, and the presence of IR-ADM in the human pituitary was studied by radioimmunoassay. ADM mRNA was clearly detected in every region of the brain examined and in the pituitary. High concentrations of IR-ADM were present in the whole pituitary (16.7 +/- 2.0 pmol/g wet weight, mean +/- SEM, n = 4). Reverse phase high performance liquid chromatography of the pituitary showed a peak eluting in the position of human ADM(1-52). These findings suggest that ADM acts as a neuromodulator or a neurotransmitter in the brain, and as an autocrine factor, a paracrine factor, or a neurohormone in the pituitary. (C) 1997 Elsevier Science Inc.

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收藏

页码：1051 / 1053

页数：3

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