Two efflux systems expressed simultaneously in multidrug-resistant Pseudomonas aeruginosa

被引：47

作者：

Pumbwe, L ^{[1
]}

Piddock, LJV ^{[1
]}

机构：

[1] Univ Birmingham, Div Immunol & Infect, Antimicrobial Agents Res Grp, Birmingham B15 2TT, W Midlands, England

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2000年 / 44卷 / 10期

关键词：

D O I：

10.1128/AAC.44.10.2861-2864.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Simultaneous overexpression of MexAB-OprM and MexEF-OprN was demonstrated for a multiply antibiotic-resistant clinical isolate of Pseudomonas aeruginosa (G49). G49 also had decreased expression of OprF. No mutations in mexR or its upstream promoter region, mexT, oprM, oprF, or sigX were revealed, suggesting regulation by a hitherto undescribed locus.

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页码：2861 / 2864

页数：4

相关论文

共 25 条

[1] Involvement of an active efflux system in the natural resistance of Pseudomonas aeruginosa to aminoglycosides [J].

Aires, JR ;

Köhler, T ;

Nikaido, H ;

Plésiat, P .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (11) :2624-2628

[2] Regulation of chromosomally mediated multiple antibiotic resistance: the mar regulon [J].

Alekshun, MN ;

Levy, SB .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (10) :2067-2075

[3] Influence of a putative ECF sigma factor on expression of the major outer membrane protein, OprF, in Pseudomonas aeruginosa and Pseudomonas fluorescens [J].

Brinkman, FSL ;

Schoofs, G ;

Hancock, REW ;

De Mot, R .

JOURNAL OF BACTERIOLOGY, 1999, 181 (16) :4746-4754

[4] A RAPID AND VERSATILE METHOD TO SYNTHESIZE INTERNAL STANDARDS FOR COMPETITIVE PCR [J].

CELI, FS ;

ZENILMAN, ME ;

SHULDINER, AR .

NUCLEIC ACIDS RESEARCH, 1993, 21 (04) :1047-1047

[5]

COHEN SP, 1988, J BACTERIOL, V170, P5146

[6] THE OUTER-MEMBRANE PROTEIN OPRM OF PSEUDOMONAS-AERUGINOSA IS ENCODED BY OPRK OF THE MEXA-MEXB-OPRK MULTIDRUG-RESISTANCE OPERON [J].

GOTOH, N ;

TSUJIMOTO, H ;

POOLE, K ;

YAMAGISHI, JI ;

NISHINO, T .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (11) :2567-2569

[7] Functional replacement of OprJ by OprM in the MexCD-OprJ multidrug efflux system of Pseudomonas aeruginosa [J].

Gotoh, N ;

Tsujimoto, H ;

Nomura, A ;

Okamoto, K ;

Tsuda, M ;

Nishino, T .

FEMS MICROBIOLOGY LETTERS, 1998, 165 (01) :21-27

[8] Characterization of the MexC-MexD-OprJ multidrug efflux system in ΔmexA-mexB-oprM mutants of Pseudomonas aeruginosa [J].

Gotoh, N ;

Tsujimoto, H ;

Tsuda, M ;

Okamoto, K ;

Nomura, A ;

Wada, T ;

Nakahashi, M ;

Nishino, T .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1998, 42 (08) :1938-1943

[9] PURIFICATION OF A 54-KILODALTON PROTEIN (OPRJ) PRODUCED IN NFXB MUTANTS OF PSEUDOMONAS-AERUGINOSA AND PRODUCTION OF A MONOCLONAL-ANTIBODY SPECIFIC TO OPRJ [J].

HOSAKA, M ;

GOTOH, N ;

NISHINO, T .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (08) :1731-1735

[10] Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa [J].

Kohler, T ;

MicheaHamzehpour, M ;

Henze, U ;

Gotoh, N ;

Curty, LK ;

Pechere, JC .

MOLECULAR MICROBIOLOGY, 1997, 23 (02) :345-354