Improved high-performance liquid chromatographic separation of peptidoglycan isolated from various Staphylococcus aureus strains for mass spectrometric characterization

被引：19

作者：

Roos, M

Pittenauer, E

Schmid, E

Beyer, M

Reinike, B

Allmaier, G

Labischinski, H

机构：

[1] Robert Koch Inst, D-13353 Berlin, Germany

[2] Univ Vienna, Inst Analyt Chem, A-1090 Vienna, Austria

[3] Bayer AG, Inst Chemotherapy, D-42096 Wuppertal, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 705卷 / 02期

关键词：

Staphylococcus aureus; peptidoglycan;

D O I：

10.1016/S0378-4347(97)00506-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Reversed-phase high-performance liquid chromatography (RP-HPLC) of muropeptides, obtained by muramidase digestion of peptidoglycan in combination with amino acid analysis and plasma desorption time-of-flight mass spectrometry is today by far the best tool to analyze the fine structure of the peptidoglycans. Here we report further improvements of the RP-HPLC separation of muropeptides for analyzing the peptidoglycans of various methicillin-resistant strains of Staphylococcus aureus, with emphasis on a more detailed characterization of the interpeptide bridge of the peptidoglycans of this species. (C) 1998 Elsevier Science B.V.

引用

页码：183 / 192

页数：10

共 19 条

[1] OPTIMIZATION OF SAMPLE DEPOSITION FOR PLASMA-DESORPTION MASS-SPECTROMETRY OF PEPTIDOGLYCAN MONOMERS [J].