Structure and function of archaeal box C/D sRNP core proteins

被引:103
作者
Aittaleb, M
Rashid, R
Chen, Q
Palmer, JR
Daniels, CJ
Li, H [1 ]
机构
[1] Florida State Univ, Inst Mol Biophys, Dept Chem & Biochem, Tallahassee, FL 32306 USA
[2] Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA
关键词
D O I
10.1038/nsb905
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nop56p and Nop58p are two core proteins of the box C/D snoRNPs that interact concurrently with fibrillarin and snoRNAs to function in enzyme assembly and catalysis. Here we report the 2.9 Angstrom resolution co-crystal structure of an archaeal homolog of Nop56p/Nop58p, Nop5p, in complex with fibrillarin from Archaeoglobus fulgidus (AF) and the methyl donor S-adenosyl-L-methionine. The N-terminal domain of Nop5p forms a complementary surface to fibrillarin that serves to anchor the catalytic subunit and to stabilize cofactor binding. A coiled coil in Nop5p mediates dimerization of two fibrillarin-Nop5p heterodimers for optimal interactions with bipartite box C/D RNAs. Structural analysis and complementary biochemical data demonstrate that the conserved C-terminal domain of Nop5p harbors RNA-binding sites. A model of box C/D snoRNP assembly is proposed based on the presented structural and biochemical data.
引用
收藏
页码:256 / 263
页数:8
相关论文
共 36 条
[1]   Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].
Brunger, AT ;
Adams, PD ;
Clore, GM ;
DeLano, WL ;
Gros, P ;
Grosse-Kunstleve, RW ;
Jiang, JS ;
Kuszewski, J ;
Nilges, M ;
Pannu, NS ;
Read, RJ ;
Rice, LM ;
Simonson, T ;
Warren, GL .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921
[2]   Coiled coils: a highly versatile protein folding motif [J].
Burkhard, P ;
Stetefeld, J ;
Strelkov, SV .
TRENDS IN CELL BIOLOGY, 2001, 11 (02) :82-88
[3]   Site-specific cross-linking analyses reveal an asymmetric protein distribution for a box C/D snoRNP [J].
Cahill, NM ;
Friend, K ;
Speckmann, W ;
Li, ZH ;
Terns, RM ;
Terns, MP ;
Steitz, JA .
EMBO JOURNAL, 2002, 21 (14) :3816-3828
[4]   AdoMet-dependent methylation, DNA methyltransferases and base flipping [J].
Cheng, XD ;
Roberts, RJ .
NUCLEIC ACIDS RESEARCH, 2001, 29 (18) :3784-3795
[5]   Box C/D RNA guides for the ribose methylation of archaeal tRNAs. The tRNATrp intron guides the formation of two ribose-methylated nucleosides in the mature tRNATrp [J].
d'Orval, BC ;
Bortolin, ML ;
Gaspin, C ;
Bachellerie, JP .
NUCLEIC ACIDS RESEARCH, 2001, 29 (22) :4518-4529
[6]   A large nucleolar U3 ribonucleoprotein required for 18S ribosomal RNA biogenesis [J].
Dragon, F ;
Gallagher, JEG ;
Compagnone-Post, PA ;
Mitchell, BM ;
Porwancher, KA ;
Wehner, KA ;
Wormsley, S ;
Settlage, RE ;
Shabanowitz, J ;
Osheim, Y ;
Beyer, AL ;
Hunt, DF ;
Baserga, SJ .
NATURE, 2002, 417 (6892) :967-970
[7]   Archaeal homologs of eukaryotic methylation guide small nucleolar RNAs:: Lessons from the Pyrococcus genomes [J].
Gaspin, C ;
Cavaillé, J ;
Erauso, G ;
Bachellerie, JP .
JOURNAL OF MOLECULAR BIOLOGY, 2000, 297 (04) :895-906
[8]   Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis [J].
Gautier, T ;
Berges, T ;
Tollervey, D ;
Hurt, E .
MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (12) :7088-7098
[9]   IMPROVED METHODS FOR BUILDING PROTEIN MODELS IN ELECTRON-DENSITY MAPS AND THE LOCATION OF ERRORS IN THESE MODELS [J].
JONES, TA ;
ZOU, JY ;
COWAN, SW ;
KJELDGAARD, M .
ACTA CRYSTALLOGRAPHICA SECTION A, 1991, 47 :110-119
[10]   Small nucleolar RNA-guided post-transcriptional modification of cellular RNAs [J].
Kiss, T .
EMBO JOURNAL, 2001, 20 (14) :3617-3622