Genomic organization of the canine herpesvirus US region

被引:20
作者
Haanes, EJ [1 ]
Tomlinson, CC [1 ]
机构
[1] Heska Corp, Ft Collins, CO 80525 USA
关键词
canine herpesvirus; pathogens; open reading frames;
D O I
10.1016/S0168-1702(97)00152-4
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Canine herpesvirus (CHV) is an alpha-herpesvirus of limited pathogenicity in healthy adult dogs and infectivity of the virus appears to be largely limited to cells of canine origin. CHV's low virulence and species specificity make it an attractive candidate for a recombinant vaccine vector to protect dogs against a variety of pathogens. As part of the analysis of the CHV genome, the authors determined the complete nucleotide sequence of the CHV US region as well as portions of the flanking inverted repeats. Seven full open reading frames (ORFs) encoding proteins larger than 100 amino acids were identified within, or partially within the CHV US: cUS2, cUS3? cUS3, cUS6, cUS7, cUS8 and cUS9; which ale homologs of the herpes simplex virus type-1 US2; protein kinase; gG, gD, gI, gE; and US9 genes, respectively. An eighth ORF was identified in the inverted repeat region, cIR6, a homolog of the equine herpesvirus type-1 IR6 gene. The authors identified and mapped most of the major transcripts for the predicted CHV US ORFs by Northern analysis, (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:151 / 162
页数:12
相关论文
共 53 条
[1]   FINE MAPPING OF BOVINE HERPESVIRUS-1 (BHV-1) GLYCOPROTEIN-D (GD) NEUTRALIZING EPITOPES BY TYPE-SPECIFIC MONOCLONAL-ANTIBODIES AND SEQUENCE COMPARISON WITH BHV-5 GD [J].
ABDELMAGID, OY ;
MINOCHA, HC ;
COLLINS, JK ;
CHOWDHURY, SI .
VIROLOGY, 1995, 206 (01) :242-253
[2]  
AURELIAN L, 1968, AM J VET RES, V29, P1945
[3]  
Ausubel FM., 1993, Current Protocols in Molecular Biology
[4]  
BINN LN, 1967, P SOC EXP BIOL MED, V126, P140, DOI 10.3181/00379727-126-32386
[5]   IDENTIFICATION AND TRANSCRIPTIONAL MAPPING OF GENES ENCODED AT THE IR US JUNCTION OF EQUINE HERPESVIRUS TYPE-1 [J].
BREEDEN, CA ;
YALAMANCHILI, RR ;
COLLE, CF ;
OCALLAGHAN, DJ .
VIROLOGY, 1992, 191 (02) :649-660
[6]   ISOLATION OF A MAREKS-DISEASE VIRUS (MDV) RECOMBINANT CONTAINING THE LACZ-GENE OF ESCHERICHIA-COLI STABLY INSERTED WITHIN THE MDV US2 GENE [J].
CANTELLO, JL ;
ANDERSON, AS ;
FRANCESCONI, A ;
MORGAN, RW .
JOURNAL OF VIROLOGY, 1991, 65 (03) :1584-1588
[7]  
CARMICHAEL LE, 1970, J AM VET MED ASSOC, V156, P1714
[8]   TRANSCRIPTIONAL ANALYSES OF THE UNIQUE SHORT SEGMENT OF EHV-1 STRAIN KENTUCKY-A [J].
COLLE, CF ;
OCALLAGHAN, DJ .
VIRUS GENES, 1995, 9 (03) :257-268
[9]   Localization of the Us protein kinase of equine herpesvirus type 1 is affected by the cytoplasmic structures formed by the novel IR6 protein [J].
Colle, CF ;
OCallaghan, DJ .
VIROLOGY, 1996, 220 (02) :424-435
[10]   IDENTIFICATION OF EQUINE HERPESVIRUS-4 GLYCOPROTEIN-G - A TYPE-SPECIFIC, SECRETED GLYCOPROTEIN [J].
CRABB, BS ;
NAGESHA, HS ;
STUDDERT, MJ .
VIROLOGY, 1992, 190 (01) :143-154