e2f1 gene is a new member of Wnt/β-catenin/Tcf-regulated genes

被引：12

作者：

Abramova, Maria V. ^{[1
]}

Zatulovskiy, Evgeny A. ^{[1
]}

Svetlikova, Svetlana B. ^{[1
]}

Kukushkin, Alexander N. ^{[1
]}

Pospelov, Valery A. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Cytol, St Petersburg 194064, Russia

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 391卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

E2F repression; HDAC inhibitor; E1A and Ras oncogenes; Wnt/beta-catenin/Tcf pathway; BETA-CATENIN; TRANSCRIPTION FACTORS; FAMILY-MEMBERS; S-PHASE; PATHWAY; PROMOTER; CELLS; ACTIVATION; INHIBITORS; ROLES;

D O I：

10.1016/j.bbrc.2009.11.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HDAC inhibitors induce cell cycle arrest of E1A + Ras-transformed cells accompanied by e2f1 gene down-regulation and activation of Writ pathway Here we show that e2f1 expression is regulated through the Wnt/Tcf-pathway: e2f1 promoter activity is inhibited by sodium butyrate (NaB) and by overexpression of beta-catenin/Tcf The e2f1 promoter was found to contain two putative Tcf-binding elements: the proximal one competes well with canonical Tcf element in DNA-binding assay Being inserted into luciferase reporter vector, the identified element provides positive transcriptional regulation in response to beta-catenin/Tcf co-transfection and NaB treatment. Thus we have firstly demonstrated that e2f1 belongs to genes regulated through Wnt/beta-catenin/Tcf pathway. (c) 2009 Elsevier Inc. All rights reserved

引用

页码：142 / 146

页数：5

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