A new method to estimate the relative bioavailability of individual amino acids in fish larvae using 13C-NMR spectroscopy

被引:21
作者
Conceiçao, LEC
Grasdalen, H
Dinis, MT
机构
[1] Univ Algarve, Ctr Ciencias Mar, CCMAR, P-8000117 Faro, Portugal
[2] NTNU, Dept Biotechnol, N-7491 Trondheim, Norway
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2003年 / 134卷 / 01期
关键词
bioavailability; indispensable amino acids; fish larvae; C-13-NMR spectroscopy;
D O I
10.1016/S1096-4959(02)00193-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In comparison to mammals, fish, and in particular young stages, are thought to have higher amino acid (AA) requirements. Still, little is known about AA requirements of fish larvae, largely due to difficulties in applying traditional methodologies to these fast growing small animals. This study presents a new method to study the qualitative AA requirements of fish larvae. This method combines the use of C-13-labelled live food and C-13-NMR spectroscopy. It allows the simultaneous estimation of the relative bioavailability of several individual AAs. The present study shows that the relative bioavailabilies of various AAs do differ between AAs in larval seabream. (Sparus aurata). Threonine has a low relative bioavailability, while aspartate, glutamate and lysine had high relative bioavailabilies compared to other AAs. These results are here attributed to differences in absorption rates by the gut, and/or selective catabolism. The results from the present study suggest that when rotifers are used as the diet for larval seabream, they should be enriched with products rich in threonine and leucine. Information on the relative bioavailability of individual AAs together with the AA profile of the larval protein should allow defining the ideal dietary AA profile for a given species. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:103 / 109
页数:7
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