The distance between P680 and QA in Photosystem II determined by ESEEM spectroscopy

被引：30

作者：

Hara, H

Dzuba, SA

Kawamori, A ^{[1
]}

Akabori, K

Tomo, T

Satoh, K

Iwaki, M

Itoh, S

机构：

[1] Kwansei Gakuin Univ, Fac Sci, Nishinomiya, Hyogo 662, Japan

[2] Russian Acad Sci, Inst Chem Kinet & Combust, Novosibirsk 630090, Russia

[3] Hiroshima Univ, Fac Integrated Arts & Sci, Higashihiroshima 739, Japan

[4] Natl Inst Basic Biol, Okazaki, Aichi 444, Japan

[5] Okayama Univ, Fac Sci, Dept Biol, Okayama 700, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1997年 / 1322卷 / 2-3期

基金：

日本学术振兴会;

关键词：

Photosystem II; P680; plastoquinone; spin-polarized radical pair; ESEEM; dipolar interaction;

D O I：

10.1016/S0005-2728(97)00081-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Light induced spin-polarized P680(+)Q(A)(-) radical pairs were studied by two pulse electron spin echo envelope modulation (ESEEM) spectroscopy in the cyanide-treated and Zn-substituted Photosystem II core complexes and in the isolated D1-D2-cyt b559 reaction center complexes reconstituted with dibromoisopropyl-p-benzoquinone. The observed strong out-of phase ESEEM signals were identified as those of the P680(+)Q(A)(-) radical pairs based on the time variation of the transient CW EPR spectra. The shapes of ESEEM spectra were attributed to dipolar D and spin exchange J interactions in the radical pairs. The values of D and J were derived from a sine Fourier transformation and the center-to-center distance between P680 and Q(A) was determined to be 27.2 +/- 1.0 Angstrom for all three preparations. (C) 1997 Elsevier Science B.V.

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页码：77 / 85

页数：9

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