Assignment of 60 human ESTs in cattle

被引：22

作者：

Laurent, P ^{[1
]}

Elduque, C ^{[1
]}

Hayes, H ^{[1
]}

Saunier, K ^{[1
]}

Eggen, A ^{[1
]}

Levéziel, H ^{[1
]}

机构：

[1] INRA, Lab Genet Biochim & Cytogenet, F-78352 Jouy En Josas, France

来源：

MAMMALIAN GENOME | 2000年 / 11卷 / 09期

关键词：

D O I：

10.1007/s003350010159

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As part of the human genome study, large-scale cDNA sequencing has produced thousands of Expressed Sequence Tags (ESTs). Genethon has mapped in human 10,000 of these ESTs and has shown that the primers of about 1000 ESTs could amplify bovine DNA. In this work, we have analyzed 233 primer pairs provided by Genethon, to assign type I sequences to the bovine genome by using a hamster-bovine somatic cell hybrid panel. Among these 233 primer pairs, 109 gave a specific PCR product with bovine genomic DNA, but for 50% the size of the FCR product was the same in cattle and hamster, requiring SSCP analysis. Finally, 60 ESTs were assigned to the bovine genome, and among them 46 were found on the bovine chromosome expected from heterologous painting data between cattle and human.

引用

页码：748 / 754

页数：7

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