A new GC-MS method for the analysis of ascaulitoxin, its aglycone and 4-aminoproline from culture filtrates of Ascochyta caulina

被引：6

作者：

Fiore, Michele ^{[2
]}

Rimando, Agnes M. ^{[2
]}

Andolfi, Anna ^{[1
]}

Evidente, Antonio ^{[1
]}

机构：

[1] Univ Naples Federico II, Dipartimento Sci Suolo Pianta Ambiente & Prod Ani, I-80055 Portici, Italy

[2] USDA ARS, Nat Prod Utilizat Res Unit, Oxford, MS 38677 USA

来源：

ANALYTICAL METHODS | 2010年 / 2卷 / 02期

关键词：

CHENOPODIUM-ALBUM; PHYTOTOXINS;

D O I：

10.1039/b9ay00162j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new GC-MS method was developed for the analysis of ascaulitoxin, its aglycone, and 4-amino-D-proline, which are phytotoxins with potential herbicidal activity produced by Ascochyta caulina. The method involved directly treating the lyophilized culture filtrate with a derivatizing reagent, converting the mixture of toxins in the filtrate to their corresponding trimethylsilyl derivatives, and consequent analysis by EI-MS. The method is rapid, sensitive and highly specific for the identification and analysis of the toxins in a complex sample matrix. Analysis of culture filtrates using this method suggested that phytotoxicity correlates with the level of ascaulitoxin in the culture filtrate. A new method for the purification of 2,4,7-triamino-5-hydroxyoctandioic acid, the aglycone of ascaulitoxin, is also described.

引用

页码：159 / 163

页数：5

共 13 条

[1] Stereochemical studies on ascaulitoxin:: a J-based NMR configurational analysis of a nitrogen substituted system [J].

Bassarello, C ;

Bifulco, G ;

Evidente, A ;

Riccio, R ;

Gomez-Paloma, L .

TETRAHEDRON LETTERS, 2001, 42 (49) :8611-8613

[2]

Davis R., 1987, MASS SPECTROMETRY

[3] MASS SPECTROMETRY IN STRUCTURAL AND STEREOCHEMICAL PROBLEMS .74. A STUDY OF FRAGMENTATION OF N-ACYLPYRROLIDINES [J].

DUFFIELD, AM ;

DJERASSI, C .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1965, 87 (20) :4554-&

[4]

Evidente A., 2006, Natural products for pest management, 226th ACS National Meeting, Anaheim, California, USA, 28 March-1 April 2004, P62

[5] Determination of Ascochyta caulina phytotoxins by high-performance anion exchange chromatography and pulsed amperometric detection [J].

Evidente, A ;

Andolfi, A ;

Vurro, M ;

Zonno, MC .

PHYTOCHEMICAL ANALYSIS, 2001, 12 (06) :383-387

[6] Ascaulitoxin, a phytotoxic bis-amino acid N-glucoside from Ascochyta caulina [J].

Evidente, A ;

Capasso, R ;

Cutignano, A ;

Taglialatela-Scafati, O ;

Vurro, M ;

Zonno, MC ;

Motta, A .

PHYTOCHEMISTRY, 1998, 48 (07) :1131-1137

[7] Trans-4-aminoproline, a phytotoxic metabolite with herbicidal activity produced by Ascochyta caulina [J].

Evidente, A ;

Andolfi, A ;

Vurro, M ;

Zonno, MC ;

Motta, A .

PHYTOCHEMISTRY, 2000, 53 (02) :231-237

[8]

Holm LG., 1977, WORLDS WORST WEEDS, P84

[9]

Kempenaar C., 1995, THESIS WAGENINGEN

[10] Biological control of Chenopodium album L. in europe [J].

Netland, J ;

Dutton, LC ;

Greaves, MP ;

Baldwin, M ;

Vurro, M ;

Evidente, A ;

Einhorn, G ;

Scheepens, PC ;

French, LW .

BIOCONTROL, 2001, 46 (02) :175-196

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