Ultrastructural localization of caspase-14 in human epidermis

被引:33
作者
Alibardi, L
Dockal, M
Reinisch, C
Tschachler, E
Eckhart, L
机构
[1] Univ Bologna, Dept Biol Evoluzionist Sperimentale, I-40126 Bologna, Italy
[2] Med Univ Vienna, Dept Dermatol, Vienna, Austria
[3] Ctr Rech & Invest Epiderm & Sensorie, Neuilly, France
关键词
human epidermis; cornification; caspase-14; ultrastructural immunocytochemistry;
D O I
10.1369/jhc.4A6300.2004
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Caspase-14 has been implicated in the formation of stratum corneum because of its specific expression and activation in terminally differentiating keratinocytes. However, its precise physiological role and its protein substrate are elusive. We studied the ultrastructural localization of caspase-14 in human epidermis to compare its distribution pattern with that of well-characterized differentiation markers. Immunogold cytochemistry confirmed that caspase-14 is nearly absent in basal and spinous layers. In the granular, layer nuclei and keratohyalin granules were labeled with increasing intensity towards the transitional layer. Particularly strong caspase-14 labeling was associated with areas known to be occupied by involucrin and loricrin, whereas F-granules, occupied by profilaggrin/filaggrin, were much less labeled. A high density of gold particles was also present at the forming cornified cell envelope, including desmosomes. In corneocytes, intense labeling was both cytoplasmic and associated with nuclear remnants and corneodesmosomes. These observations will allow focusing efforts of biochemical substrate screening on a subset of proteins localizing to distinct compartments of terminally differentiated keratinocytes.
引用
收藏
页码:1561 / 1574
页数:14
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