Effect of caged fluorescent dye on the electroosmotic mobility in microchannels

被引：38

作者：

Ross, D ^{[1
]}

Locascio, LE

机构：

[1] NIST, Proc Measurement Div, Gaithersburg, MD 20899 USA

[2] NIST, Div Analyt Chem, Gaithersburg, MD 20899 USA

来源：

ANALYTICAL CHEMISTRY | 2003年 / 75卷 / 05期

关键词：

D O I：

10.1021/ac026277u

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report on measurements of electroosmotic mobility in polymer microchannels and silica capillaries with and without the addition of a caged fluorescein dye to the buffer. For PMMA microchannels, the mobility was found to increase from (2.6 +/- 0. 1) x 10(-4) cm(2) V-1 s(-1) to (4.6 +/- 0.1) x 10(-4) cm(2) V-1 s(-1) upon addition of 1.2 mmol/L of caged dye. For PC microchannels, the mobility increased from (4.3 +/- 0.2) x 10(-4) cm(2) V-1 s(-1) to (5.4 +/- 0. 1) x 10(-4) cm(2) V-1 s(-1) upon addition of caged dye. For PDMS microchannels, the mobility increased from (4.3 +/- 0.2) x 10(-4) cm(2) V-1 s(-1) to (6.4 +/- 10.5) x 10(-4) cm(2) V-1 s(-1) upon addition of caged dye. For fused-silica capillaries, the mobility ((5.5 +/- 0.2) x 10(-4) cm(2) V-1 s(-1)) was unaffected by the addition of the caged dye.

引用

页码：1218 / 1220

页数：3

共 17 条

[1] CAPILLARY ELECTROPHORESIS AND SAMPLE INJECTION SYSTEMS INTEGRATED ON A PLANAR GLASS CHIP [J].