Antibodies directed at mouse IL-2-R alpha and beta chains act in synergy to abolish T-cell proliferation in vitro and delayed type hypersensitivity reaction in vivo

被引：4

作者：

Francois, C ^{[1
]}

Dantal, J ^{[1
]}

Sorel, M ^{[1
]}

Cherel, M ^{[1
]}

Miyasaka, M ^{[1
]}

Soulillou, JP ^{[1
]}

Jacques, Y ^{[1
]}

机构：

[1] INST BIOL,INSERM,U211,F-44035 NANTES 01,FRANCE

来源：

TRANSPLANT INTERNATIONAL | 1996年 / 9卷 / 01期

关键词：

monoclonal antibodies; mouse interleukin-2 receptor;

D O I：

10.1007/BF00336811

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

The anti-mouse IL-2-R beta chain mAb TM-beta 1 which by itself, does not affect IL-2-dependent proliferation through the high affinity mouse IL-2 receptor, was shown to cooperate in a synergistic way with a set of anti-IL-2-R alpha chain mAbs both in vitro and in vivo. In vitro, when associated at equimolar concentrations, the TM-beta 1/anti-alpha mAb association was four to ten times more efficient at inhibiting the proliferation of the CTL-L2 cell lint than was a similar concentration of anti-alpha mAb alone. In addition, a bispecific antibody in which a Fab' fragment of TM-beta 1 was covalently linked to a Fab' fragment of one of the anti-alpha mAb (5A2) was shown to be as efficient as the TM-beta 1/5A2 association. The association of TM-beta 1 with 5A2 was also tested in vivo in a sheep red blood cell-induced delayed type hypersensitivity (DTH) model. TM-beta 1 which. by itself, had no effect on DTH, induced a two- to threefold decrease in the doses of 5A2 required to suppress this cell-mediated immune reaction.

引用

页码：46 / 50

页数：5