Preparation of poly(lactic acid)/chitosan nanoparticles for anti-HIV drug delivery applications

被引:182
作者
Dev, Ashish [1 ,2 ]
Binulal, N. S. [1 ,2 ]
Anitha, A. [1 ,2 ]
Nair, S. V. [1 ,2 ]
Furuike, T. [3 ,4 ]
Tamura, H. [3 ,4 ]
Jayakumar, R. [1 ,2 ]
机构
[1] Amrita Vishwa Vidhyapeetham Univ, Amrita Ctr Nanosci & Mol Med, Amrita Inst Med Sci, Cochin 682041, Kerala, India
[2] Amrita Vishwa Vidhyapeetham Univ, Res Ctr, Cochin 682041, Kerala, India
[3] Kansai Univ, Fac Chem Mat & Bioengn, Osaka 5648680, Japan
[4] Kansai Univ, High Technol Res Ctr, Osaka 5648680, Japan
关键词
Poly(lactic acid); Chitosan; Lamivudine drug; Emulsion; Nanoparticles; Controlled drug delivery; CHITOSAN; DEGRADATION; MICROSPHERES; SCAFFOLDS; BEHAVIOR; RELEASE;
D O I
10.1016/j.carbpol.2009.12.040
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Poly(lactic acid) (PLA)/chitosan (CS) nanoparticles were prepared by emulsion method for anti-HIV drug delivery applications. The prepared PLA/CS nanoparticles were characterized using DLS, SEM, and FTIR. The hydrophilic antiretroviral drug Lamivudine was loaded into PLA/CS nanoparticles. The encapsulation efficiency and in-vitro drug release behaviour of drug loaded PLA/CS nanoparticles were studied using UV spectrophotometer. In addition, the cytotoxicity of the PLA/CS nanoparticles using MTT assay was also studied. The in-vitro drug release studies showed that drug release rate was lower in the acidic pH when compared to alkaline pH. This may due to repulsion between H ions and cationic groups present in the polymeric nanoparticles. Drug release rate was found to be higher in the 6% drug loaded formulation when compared to 6% drug loaded formulation. These results indicated that the PLA/CS nanoparticles are a promising carrier system for controlled delivery of anti-HIV drugs. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:833 / 838
页数:6
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