The effectiveness of passive ultrasonic irrigation on intraradicular Enterococcus faecalis biofilms in extracted single-rooted human teeth

被引:63
作者
Bhuva, B. [1 ]
Patel, S. [1 ]
Wilson, R. [2 ]
Niazi, S. [3 ]
Beighton, D. [3 ]
Mannocci, F. [1 ]
机构
[1] Kings Coll London, Inst Dent, Dept Conservat Dent, London SE1 9RT, England
[2] Kings Coll London, Inst Dent, Dept Periodontol, London SE1 9RT, England
[3] Kings Coll London, Inst Dent, Dept Microbiol, London WC2R 2LS, England
关键词
biofilms; conventional syringe irrigation; Enterococcus faecalis; intraradicular; passive ultrasonic irrigation; scanning electron microscopy; PLACED DENTIN DEBRIS; HUMAN MANDIBULAR MOLARS; IN-VITRO; APICAL PERIODONTITIS; SODIUM-HYPOCHLORITE; BACTERIOLOGIC EVALUATION; ENDODONTIC THERAPY; PERIAPICAL LESIONS; CANAL IRRIGATION; INSTRUMENTATION;
D O I
10.1111/j.1365-2591.2009.01672.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
P>Aim To compare the efficacy of passive ultrasonic irrigation with 1% sodium hypochlorite, with that of conventional syringe irrigation with 1% sodium hypochlorite, on intraradicular Enterococcus faecalis biofilms in extracted single-rooted human teeth. Methodology Biofilms of E. faecalis (strain OMGS 3202) were grown on the prepared root canal walls of 48 standardized root halves which had been longitudinally sectioned. Following reapproximation, the roots were divided into four groups of twelve. The two experimental groups were treated with conventional syringe irrigation with 1% sodium hypochlorite solution (experimental group A) and passive ultrasonic irrigation with 1% sodium hypochlorite solution (experimental group B). Of the two control groups, the first was treated with conventional syringe irrigation with sterile saline solution (control group C), whilst the second control group (D) received no irrigation. The root halves were processed for scanning electron microscopy. Three images (x700), coronal, middle and apical, were taken of the twelve root halves in each of the four groups, using a standardized protocol. The images were randomized and biofilm coverage assessed independently by three calibrated examiners, using a four-point scoring system. Results There were no significant differences in the scores for remaining biofilm coverage between group A (conventional syringe irrigation with 1% sodium hypochlorite) and group B (passive ultrasonic irrigation with 1% sodium hypochlorite) at the three observed levels. There was a significant difference between both experimental groups (groups A and B) and group C (conventional syringe irrigation with sterile saline solution) (P < 0.001) at all three observed levels. Conclusions Both conventional syringe irrigation and passive ultrasonic irrigation with 1% sodium hypochlorite were effective at completely removing intraradicular E. faecalis biofilms. Conventional syringe irrigation with sterile saline solution was only partially effective at removing the biofilms.
引用
收藏
页码:241 / 250
页数:10
相关论文
共 49 条
[1]   Acoustic Hypochlorite Activation in Simulated Curved Canals [J].
Al-Jadaa, Anas ;
Paque, Frank ;
Attin, Thomas ;
Zehnder, Matthias .
JOURNAL OF ENDODONTICS, 2009, 35 (10) :1408-1411
[2]   Evaluation of apical debris removal using various sizes and tapers of ProFile GT files [J].
Albrecht, LJ ;
Baumgartner, JC ;
Marshall, JG .
JOURNAL OF ENDODONTICS, 2004, 30 (06) :425-428
[3]   Coronal leakage of mixed anaerobic bacteria after obturation and post space preparation [J].
Barrieshi, KM ;
Walton, RE ;
Johnson, WT ;
Drake, DR .
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS, 1997, 84 (03) :310-314
[4]   The in vivo evaluation of hand/rotary/ultrasound instrumentation in necrotic, human mandibular molars [J].
Burleson, Aaron ;
Nusstein, John ;
Reader, Al ;
Beck, Mike .
JOURNAL OF ENDODONTICS, 2007, 33 (07) :782-787
[5]  
BYSTROM A, 1981, SCAND J DENT RES, V89, P321
[6]   BACTERIOLOGIC EVALUATION OF THE EFFECT OF 0.5 PERCENT SODIUM-HYPOCHLORITE IN ENDODONTIC THERAPY [J].
BYSTROM, A ;
SUNDQVIST, G .
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 1983, 55 (03) :307-312
[7]   IMAGING OF BACTERIAL-CELLS BY FLUORESCENCE EXCLUSION USING SCANNING CONFOCAL LASER MICROSCOPY [J].
CALDWELL, DE ;
KORBER, DR ;
LAWRENCE, JR .
JOURNAL OF MICROBIOLOGICAL METHODS, 1992, 15 (04) :249-261
[8]   MECHANICAL EFFECTIVENESS OF ROOT-CANAL IRRIGATION [J].
CHOW, TW .
JOURNAL OF ENDODONTICS, 1983, 9 (11) :475-479
[9]   The effect of exposure to irrigant solutions on apical dentin biofilms in vitro [J].
Clegg, MS ;
Vertucci, FJ ;
Walker, C ;
Britto, LR .
JOURNAL OF ENDODONTICS, 2006, 32 (05) :434-437
[10]  
DALTON BC, 1998, J ENDODONT, V24, P1