A freezing-sensitive mutant of Arabidopsis, frs1, is a new aba3 allele

被引:44
作者
Llorente, F [1 ]
Oliveros, JC [1 ]
Martínez-Zapater, JM [1 ]
Salinas, J [1 ]
机构
[1] Inst Nacl Invest & Tecnol Agr & Alimentaria, Dept Mejora Genet & Biotecnol, E-28040 Madrid, Spain
关键词
abscisic acid; Arabidopsis (mutant frs1); cold acclimation; dehydration; freezing tolerance; frs1; mutant;
D O I
10.1007/s004250000340
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
To investigate the molecular mechanisms controlling the process of cold acclimation and to identify genes involved in plant freezing tolerance, mutations that impaired the cold acclimation capability of Arabidopsis thaliana (L.) Heynh. were screened for. A new mutation, frs1 (freezing sensitive 1), that reduced both the constitutive freezing tolerance as well as the freezing tolerance of Arabidopsis after cold acclimation was characterized. This mutation also produced a wilty phenotype and excessive water loss. Plants with the frs1 mutation recovered their wild-type phenotype, their capability to tolerate freezing temperatures and their capability to retain water after an exogenous abscisic acid (ABA) treatment. Measurements of ABA revealed that frs1 mutants were ABA deficient, and complementation tests indicated that frs1 mutation was a new allele of the ABAS locus showing that a mutation in this locus leads to an impairment of freezing tolerance. These results constitute the first report showing that a mutation in ABA3 leads to an impairment of freezing tolerance, and not only strengthen the conclusion that ABA is required for full development of freezing tolerance in cold-acclimated plants, but also demonstrate that ABA mediates the constitutive freezing tolerance of Arabidopsis. Gene expression in frs1 mutants was altered in response to dehydration, suggesting that freezing tolerance in Arabidopsis depends on ABA-regulated proteins that allow plants to survive the challenges imposed by subzero temperatures, mainly freeze-induced cellular dehydration.
引用
收藏
页码:648 / 655
页数:8
相关论文
共 47 条
[1]   Constitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance [J].
Artus, NN ;
Uemura, M ;
Steponkus, PL ;
Gilmour, SJ ;
Lin, CT ;
Thomashow, MF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (23) :13404-13409
[2]   ASSIGNMENT OF 30 MICROSATELLITE LOCI TO THE LINKAGE MAP OF ARABIDOPSIS [J].
BELL, CJ ;
ECKER, JR .
GENOMICS, 1994, 19 (01) :137-144
[4]   Two homologous low-temperature-inducible genes from Arabidopsis encode highly hydrophobic proteins [J].
Capel, J ;
Jarillo, JA ;
Salinas, J ;
MartinezZapater, JM .
PLANT PHYSIOLOGY, 1997, 115 (02) :569-576
[5]   RAPID EXTRACTION OF FUNGAL DNA FOR PCR AMPLIFICATION [J].
CENIS, JL .
NUCLEIC ACIDS RESEARCH, 1992, 20 (09) :2380-2380
[6]   ABSCISIC ACID-INDUCED FREEZING RESISTANCE IN CULTURED PLANT-CELLS [J].
CHEN, THH ;
GUSTA, LV .
PLANT PHYSIOLOGY, 1983, 73 (01) :71-75
[7]   COLD-ACCLIMATION AND COLD-REGULATED GENE-EXPRESSION IN ABA MUTANTS OF ARABIDOPSIS-THALIANA [J].
GILMOUR, SJ ;
THOMASHOW, MF .
PLANT MOLECULAR BIOLOGY, 1991, 17 (06) :1233-1240
[8]   CDNA SEQUENCE-ANALYSIS AND EXPRESSION OF 2 COLD-REGULATED GENES OF ARABIDOPSIS-THALIANA [J].
GILMOUR, SJ ;
ARTUS, NN ;
THOMASHOW, MF .
PLANT MOLECULAR BIOLOGY, 1992, 18 (01) :13-21
[9]   Leaf abscission induced by ethylene in water-stressed intact seedlings of cleopatra mandarin requires previous abscisic acid accumulation in roots [J].
GomezCadenas, A ;
Tadeo, FR ;
Talon, M ;
PrimoMillo, E .
PLANT PHYSIOLOGY, 1996, 112 (01) :401-408
[10]   DETECTION OF POLYPEPTIDES ASSOCIATED WITH THE COLD-ACCLIMATION PROCESS IN SPINACH [J].
GUY, CL ;
HASKELL, D .
ELECTROPHORESIS, 1988, 9 (11) :787-796