Dual bio-responsive gene delivery via reducible poly(amido amine) and survivin-inducible plasmid DNA

被引：12

作者：

Namgung, Ran ^{[1
]}

Brumbach, Jonathan H. ^{[2
]}

Jeong, Ji Hoon ^{[3
]}

Yockman, James W. ^{[2
]}

Kim, Sung Wan ^{[2
]}

Lin, Chao ^{[4
,5
]}

Zhong, Zhiyuan ^{[6
,7
]}

Feijen, Jan ^{[4
,5
]}

Engbersen, Johan F. J. ^{[4
,5
]}

Kim, Won Jong ^{[1
]}

机构：

[1] Pohang Univ Sci & Technol, Dept Chem, Pohang 790784, South Korea

[2] Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Ctr Controlled Chem Delivery, Salt Lake City, UT 84112 USA

[3] Sungkyunkwan Univ, Coll Pharm, Suwon 440746, South Korea

[4] Univ Twente, Dept Polymer Chem & Biomat, Fac Sci & Technol, NL-7500 AE Enschede, Netherlands

[5] Univ Twente, Inst Biomed Technol, NL-7500 AE Enschede, Netherlands

[6] Soochow Univ, Biomed Polymers Lab, Coll Chem Chem Engn & Mat Sci, Suzhou 215123, Peoples R China

[7] Soochow Univ, Key Lab Organ Synth Jiangsu Prov, Coll Chem Chem Engn & Mat Sci, Suzhou 215123, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 06期

关键词：

Dual bio-responsive; Gene therapy; Reducible polymer; Survivin; Tumor cells; ONCOLYTIC ADENOVIRUS; DISULFIDE LINKAGES; MOUSE SURVIVIN; CANCER; APOPTOSIS; PROMOTER; INHIBITION; EXPRESSION; COMPLEXES; THERAPY;

D O I：

10.1007/s10529-010-0219-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A bioreducible poly(amido amine) (SS-PAA) gene carrier, known as poly (amido-butanol) (pABOL), was used to transfect a variety of cancer and non-cancer cell lines. To obtain cancer-specific transgene expression for therapeutic efficiency in cancer treatment, we constructed survivin-inducible plasmid DNA expressing the soluble VEGF receptor, sFlt-1, downstream of the survivin promoter (pSUR-sFlt-1). Cancer-specific expression of sFlt-1 was observed in the mouse renal carcinoma (RENCA) cell line. pABOL enhanced the efficiency of gene delivery compared to traditional carriers used in the past. Thus, a dual bio-responsive gene delivery system was developed by using bioreducible p(ABOL) for enhanced intracellular gene delivery and survivin-inducible gene expression system (pSUR-sFlt-1 or pSUR-Luc reporter gene) that demonstrates increased gene expression in cancer that has advantages over current gene delivery systems.

引用

页码：755 / 764

页数：10

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