Phenotypic and Proteomic Characteristics of Human Dental Pulp Derived Mesenchymal Stem Cells from a Natal, an Exfoliated Deciduous, and an Impacted Third Molar Tooth

被引:50
作者
Akpinar, Gurler [1 ]
Kasap, Murat [1 ,2 ]
Aksoy, Ayca [3 ]
Duruksu, Gokhan [3 ]
Gacar, Gulcin [3 ]
Karaoz, Erdal [3 ,4 ]
机构
[1] Kocaeli Univ, Sch Med, DEKART Prote Lab, TR-41380 Umuttepe, Kocaeli, Turkey
[2] Kocaeli Univ, Sch Med, Dept Med Biol, TR-41380 Umuttepe, Kocaeli, Turkey
[3] Inst Hlth Sci, Ctr Stem Cell & GeneTherapies Res & Practice, Dept Stem Cell, TR-41380 Umuttepe, Kocaeli, Turkey
[4] Liv Hosp, Ctr Regenerat Med & Stem Cell Res & Mfg Liv MedCe, TR-34340 Istanbul, Turkey
关键词
PROLYL ISOMERASE PIN1; BONE-MARROW; DIFFERENTIATION; TELOMERASE; RADIOPROTECTION; IDENTIFICATION; PROLIFERATION; HETEROGENEITY; POPULATIONS; CALDESMON;
D O I
10.1155/2014/457059
中图分类号
Q813 [细胞工程];
学科分类号
100113 [医学细胞生物学];
摘要
The level of heterogeneity among the isolated stem cells makes them less valuable for clinical use. The purpose of this study was to understand the level of heterogeneity among human dental pulp derived mesenchymal stem cells by using basic cell biology and proteomic approaches. The cells were isolated from a natal (NDPSCs), an exfoliated deciduous (stem cells from human exfoliated deciduous (SHED)), and an impacted third molar (DPSCs) tooth of three different donors. All three stem cells displayed similar features related to morphology, proliferation rates, expression of various cell surface markers, and differentiation potentials into adipocytes, osteocytes, and chondrocytes. Furthermore, using 2DE approach coupled with MALDI-TOF/TOF, we have generated a common 2DE profile for all three stem cells. We found that 62.3 +/- 7% of the protein spots were conserved among the three mesenchymal stem cell lines. Sixty-one of these conserved spots were identified by MALDI-TOF/TOF analysis. Classification of the identified proteins based on biological function revealed that structurally important proteins and proteins that are involved in protein folding machinery are predominantly expressed by all three stem cell lines. Some of these proteins may hold importance in understanding specific properties of human dental pulp derived mesenchymal stem cells.
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页数:19
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