Hemoglobin depletion from red blood cell cytosol reveals new proteins in 2-D gel-based proteomics study

被引：52

作者：

Bhattacharya, Dipankar

Mukhopadhyay, Debashis

Chakrabarti, Abhijit

机构：

[1] Saha Inst Nucl Phys, Div Biophys, Kolkata 700064, W Bengal, India

[2] Saha Inst Nucl Phys, Struct Genom Sect, Kolkata 700064, W Bengal, India

来源：

PROTEOMICS CLINICAL APPLICATIONS | 2007年 / 1卷 / 06期

关键词：

2-D gel; depletion; hemoglobin; red blood cells;

D O I：

10.1002/prca.200700178

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Proteomics studies to identify proteins in the erythrocyte cytosol have been largely affected by the huge abundance of hemoglobin (Hb), which masks the detection of other proteins in the 2-D gel-based separation. We have depleted Hb effectively from erythrocyte cytosol using cation exchange chromatography and have detected more than 600 protein spots in the Hb depleted hemolysate using 2-DE. We have so far identified 59 proteins in the Hb-depleted cytosol of normal erythrocytes, including 10 proteins not identified before.

引用

收藏

页码：561 / 564

页数：4

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