Induction of DNA replication-mediated double strand breaks by psoralen DNA interstrand cross-links

被引：77

作者：

Bessho, T ^{[1
]}

机构：

[1] Univ Texas, Hlth Sci Ctr, Inst Biotechnol, Dept Mol Med, San Antonio, TX 78245 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2003年 / 278卷 / 07期

关键词：

D O I：

10.1074/jbc.M212323200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of DNA interstrand cross-links (cross-links) on DNA replication was examined with a cell-free SV40 origin-dependent DNA replication system. A defined template DNA with a single psoralen cross-link and the SV40 origin of replication was replicated by HeLa cell-free extract in the presence of SV40 large T antigen. The psoralen cross-link inhibited DNA replication by terminating chain elongation at 1-50 nucleotides before the cross-linked sites. The termination of DNA replication by the cross-links mediated the generation of double strand breaks near the cross-linked sites. These results are the first biochemical evidence of the generation of double strand breaks by DNA replication.

引用

页码：5250 / 5254

页数：5

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