Effects of Hoechst 33342 on C2C12 and PC12 cell differentiation

被引：26

作者：

Adamski, Daniele

Mayol, Jean-Francois

Plateta, Nadine

Berger, Francois

Herodin, Francis

Wion, Didier ^{[1
]}

机构：

[1] CHU Michallon, INSERM, U836, F-38043 Grenoble, France

[2] Ctr Rech, Serv Sante Armees, F-38702 La Tronche, France

来源：

FEBS LETTERS | 2007年 / 581卷 / 16期

关键词：

side population; Hoechst; 33342; cancer stem cell; stem cell;

D O I：

10.1016/j.febslet.2007.05.073

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Accumulative evidence demonstrates that normal as well as cancer stem cells can be identified as a side population following Hoechst 33342 staining and flow cytometric analysis. This popular method is based on the ability of stem cells to efflux this fluorescent vital dye. We demonstrate that Hoechst 33342 can affect cell differentiation, suggesting potential complications in the interpretation of data. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：3076 / 3080

页数：5

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