Spectrofluorimetric determination of aliphatic amines using a new fluorigenic reagent:: 2,6-dimethylquinoline-4-(N-succinimidyl)-formate

被引：22

作者：

Cao, LW ^{[1
]}

Wang, H ^{[1
]}

Liu, X ^{[1
]}

Zhang, HS ^{[1
]}

机构：

[1] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

来源：

TALANTA | 2003年 / 59卷 / 05期

基金：

高等学校博士学科点专项科研基金; 中国国家自然科学基金;

关键词：

2,6-dimethylquinoline-4-(N-succinimidyl) formate; spectrofluorimetry; aliphatic amines;

D O I：

10.1016/S0039-9140(03)00008-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new amino fluorescence probe, 2,6-dimethylquinoline-4-(N-succinimidyl) formate (DMQF-OSu) has been synthesized. Based on the selective reaction of DMQF-OSu with primary and secondary aliphatic amines to yield strong fluorescence, a new spectrofluorimetric method for the determination of total aliphatic amines has been developed. At lambda(ex)/lambda(em) 324.4/416 nm, the linear calibration range was 6 x 10(-8) -6 x 10(-6) Mol 1(-1) with the detection limit (3sigma) of 1.94 x 10(-10) mol 1-1 for the determination of aliphatic amines in weak basic media. The proposed method has been applied to the determination of aliphatic amines in tap water and lake water with the recoveries of 99-104%. Compared with the reported methods, the method presented here is rapid, simple, sensitive and feasible. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：973 / 979

页数：7

共 19 条

[1] Analysis of amines in plant materials [J].