An enantioselective fluorimetric assay for alcohol dehydrogenases using albumin-catalyzed β-elimination of umbelliferone

被引：70

作者：

Klein, G ^{[1
]}

Reymond, JL ^{[1
]}

机构：

[1] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1998年 / 8卷 / 09期

关键词：

D O I：

10.1016/S0960-894X(98)00165-6

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

3-hydroxybutyl umbelliferyl ethers (R)-1 and (S)-1 are fluorogenic substrates for alcohol dehydrogenases. Their oxidation forms ketone 2, which undergoes beta-elimination of umbelliferone under catalysis by bovine serum albumin, leading to a >20-fold fluorescence increase at lambda(em) = 460 +/- 20 nm (lambda(ex) = 360 +/- 20 nm). Enantioselectivity is determined in two separate tests with each enantiomeric substrate. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：1113 / 1116

页数：4

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