Simulations of the effects of inositol 1,4,5-trisphosphate 3-kinase and 5-phosphatase activities on Ca2+ oscillations

被引:74
作者
Dupont, G
Erneux, C
机构
[1] Free Univ Brussels, Fac Sci, Unite Chronobiol Theor, B-1050 Brussels, Belgium
[2] Free Univ Brussels, Fac Med, Inst Rech Interdisciplinaire, B-1050 Brussels, Belgium
关键词
D O I
10.1016/S0143-4160(97)90017-8
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Inositol 1,4,5-trisphosphate (Ins-1,4,5-P-3) is responsible for Ca2+ mobilization in response to external stimulation in many cell types. The latter phenomenon often occurs as repetitive Ca2+ spikes. In this study, the effect of the two Ins-1,4,5-P-3 metabolizing enzymes (Ins-1,4,5-P-3 3-kinase and 5-phosphatase) on the temporal pattern of Ca2+ oscillations has been investigated. On the basis of the well-documented Ins-1,4,5-P-3 3-kinase stimulation by the Ca2+/calmodulin complex and of the experimentally-determined kinetic characteristics of these enzymes, we predict that 5-phosphatase primarily controls the levels of Ins-1,4,5-P-3 and, thereby, the occurrence and frequency of Ca2+ oscillations. Consequently, the model reproduces the experimental observation performed in Chinese hamster ovary cells that 5-phosphatase overexpression has a much more pronounced effect on the pattern of Ca2+ oscillations than 3-kinase overexpression. We also investigated, in more detail, under which conditions a similar effect could be observed in other cell types expressing various Ins-1,4,5-P-3 3-kinase activities.
引用
收藏
页码:321 / 331
页数:11
相关论文
共 54 条
[1]   A SINGLE-POOL MODEL FOR INTRACELLULAR CALCIUM OSCILLATIONS AND WAVES IN THE XENOPUS-LAEVIS OOCYTE [J].
ATRI, A ;
AMUNDSON, J ;
CLAPHAM, D ;
SNEYD, J .
BIOPHYSICAL JOURNAL, 1993, 65 (04) :1727-1739
[2]  
BALLA T, 1991, J BIOL CHEM, V266, P24719
[3]   INOSITOL TRISPHOSPHATE AND CALCIUM SIGNALING [J].
BERRIDGE, MJ .
NATURE, 1993, 361 (6410) :315-325
[4]   BELL-SHAPED CALCIUM-RESPONSE CURVES OF INS(1,4,5)P3-GATED AND CALCIUM-GATED CHANNELS FROM ENDOPLASMIC-RETICULUM OF CEREBELLUM [J].
BEZPROZVANNY, I ;
WATRAS, J ;
EHRLICH, BE .
NATURE, 1991, 351 (6329) :751-754
[5]   Effect of inositol 1,3,4,5-tetrakisphosphate on inositol trisphosphate-activated Ca2+ signaling in mouse lacrimal acinar cells [J].
Bird, GSJ ;
Putney, JW .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (12) :6766-6770
[6]   Complex intracellular calcium oscillations - A theoretical exploration of possible mechanisms [J].
Borghans, JAM ;
Dupont, G ;
Goldbeter, A .
BIOPHYSICAL CHEMISTRY, 1997, 66 (01) :25-41
[7]   MOLECULAR-CLONING AND EXPRESSION OF A COMPLEMENTARY-DNA FOR INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE [J].
CHOI, KY ;
KIM, HK ;
LEE, SY ;
MOON, KH ;
SIM, SS ;
KIM, JW ;
CHUNG, HK ;
RHEE, SG .
SCIENCE, 1990, 248 (4951) :64-66
[8]   PURIFICATION AND BIOCHEMICAL-PROPERTIES OF A HIGH-MOLECULAR-MASS INOSITOL 1,4,5-TRISPHOSPHATE 3-KINASE ISOENZYME IN HUMAN PLATELETS [J].
COMMUNI, D ;
VANWEYENBERG, V ;
ERNEUX, C .
BIOCHEMICAL JOURNAL, 1994, 298 :669-673
[9]   D-myo-inositol 1,4,5-trisphosphate 3-kinase A is activated by receptor activation through a calcium:calmodulin-dependent protein kinase II phosphorylation mechanism [J].
Communi, D ;
Vanweyenberg, V ;
Erneux, C .
EMBO JOURNAL, 1997, 16 (08) :1943-1952
[10]   MODELING RECEPTOR-CONTROLLED INTRACELLULAR CALCIUM OSCILLATORS [J].
CUTHBERTSON, KSR ;
CHAY, TR .
CELL CALCIUM, 1991, 12 (2-3) :97-109