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Aminopeptidase C of Aspergillus niger is a novel phenylalanine aminopeptidase

被引：13
作者：
Basten, DEJW
Dekker, PJT
Schaap, PJ
机构：
[1] Univ Wageningen & Res Ctr, Microbiol Lab, NL-6703 HA Wageningen, Netherlands
[2] DSM Food Specialties, NL-2600 MA Delft, Netherlands
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2003年 / 69卷 / 02期
关键词：
D O I：
10.1128/AEM.69.2.1246-1250.2003
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];
摘要：
A novel enzyme with a specific phenylalanine aminopeptidase activity (ApsC) from Aspergillus niger (CBS 120.49) has been characterized. The derived amino acid sequence is not similar to any previously characterized aminopeptidase sequence but does share similarity with some mammalian acyl-peptide hydrolase sequences. ApsC was found to be most active towards phenylalanine beta-naphthylamide (F-betaNA) and phenylalanine para-nitroanilide (F-betaNA), but it also displayed activity towards other amino acids with aromatic side chains coupled to betaNA; other amino acids with nonaromatic side chains coupled to either pNA or betaNA were not hydrolyzed or were poorly hydrolyzed. ApsC was not able to hydrolyze N-acetylalanine-pNA, a substrate for acyl-peptide hydrolases.
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页码：1246 / 1250
页数：5
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