Structure and specific expression of a Nicotiana sylvestris putative amino-acid transporter gene in mature and in vitro germinating pollen.

被引：15

作者：

Lalanne, E ^{[1
]}

Mathieu, C ^{[1
]}

Roche, O ^{[1
]}

Vedel, F ^{[1
]}

De Pape, R ^{[1
]}

机构：

[1] Univ Paris 11, ERS 569, Inst Biotechnol Plantes, F-91405 Orsay, France

来源：

PLANT MOLECULAR BIOLOGY | 1997年 / 35卷 / 06期

关键词：

amino acid transporter; gene sequence; in vitro pollen germination; in situ hybridization; Nicotiana sylvestris; pollen-specific expression;

D O I：

10.1023/A:1005812419151

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here the structure and expression analysis of a Nicotiana sylvestris gene, called nsaap1, coding for a protein which shows significant identity to the members of the Arabidopsis thaliana amino acid permease family. The nsaap1 gene is expressed exclusively in binucleate pollen. Its transcripts begin to accumulate after first pollen mitosis and dramatically increase in mature pollen shortly before anthesis and during in vitro pollen germination. The nsaap1 5'-flanking region contains long regions homologous to the promoter region of the tobacco pollen specific elF-4A8 translation factor. Our results support the occurrence of an amino acid remobilization in binucleate pollen. The nsaap1 gene could play a role in exchanges between the vegetative and the generative pollen cells.

引用

页码：855 / 864

页数：10

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