Regulation of tyrosine hydroxylase gene transcription by Sry

被引:62
作者
Milsted, A [1 ]
Serova, L
Sabban, EL
Dunphy, G
Turner, ME
Ely, DL
机构
[1] Univ Akron, Dept Biol, Akron, OH 44325 USA
[2] New York Med Coll, Dept Biochem & Mol Biol, Valhalla, NY 10595 USA
关键词
tyrosine hydroxylase; promoter; gene expression; APl element; Sry; Y chromosome; gender differences;
D O I
10.1016/j.neulet.2004.07.052
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Testes determining factor Sry is encoded by the Sry locus on the Y chromosome and may be involved in the regulation of blood pressure. Here we tested the hypothesis that Sry regulates transcription of tyrosine hydroxylase (TH), the rate-limiting enzyme in the biosynthesis of catecholamines. Sry was found to be expressed in catecholaminergic regions, in male but not female rats. Co-transfection of PC12 cells with expression vector for Sry and the reporter construct [p5'TH(-773/+27)/Luc], containing 773 of the proximal nucleotides of the TH promoter directing luciferase reporter activity, led to elevation of reporter activity. The reporter activity of a shorter construct [p5'TH(-272/+27)/Luc] lacking putative Sry sites also responded to Sty. However, mutation of the AP1 site in the TH promoter greatly reduced induction by Sty, indicating that the regulation is primarily at this motif. The remaining, significantly increased expression with the mutated TH promoter construct may reflect Sry function at other sites in addition to the AP1 motif. These results reveal that Sry can regulate TH transcription and suggest that this may be one of the mechanisms of Sry mediated regulation of catecholamine biosynthesis in catecholaminergic neurons in males. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:203 / 207
页数:5
相关论文
共 32 条
[1]  
ASTONJONES G, 1984, NOREPINEPHRINE
[2]  
BERRUDGEN CW, 2003, BRAIN RES REV, P42
[3]   SHR Y chromosome enhances the nocturnal blood pressure in socially interacting rats [J].
Caplea, A ;
Seachrist, D ;
Dunphy, G ;
Ely, D .
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2000, 279 (01) :H58-H66
[4]   Noradrenergic content and turnover rate in kidney and heart shows gender and strain differences [J].
Caplea, A ;
Seachrist, D ;
Daneshvar, H ;
Dunphy, G ;
Ely, D .
JOURNAL OF APPLIED PHYSIOLOGY, 2002, 92 (02) :567-571
[5]   SRY IS A TRANSCRIPTIONAL ACTIVATOR [J].
DUBIN, RA ;
OSTRER, H .
MOLECULAR ENDOCRINOLOGY, 1994, 8 (09) :1182-1192
[6]   Review of the Y chromosome and hypertension [J].
Ely, D ;
Turner, M ;
Milsted, A .
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, 2000, 33 (06) :679-691
[7]   Spontaneously hypertensive rat Y chromosome increases indexes of sympathetic nervous system activity [J].
Ely, D ;
Caplea, A ;
Dunphy, G ;
Daneshvar, H ;
Turner, M ;
Milsted, A ;
Takiyyuddin, M .
HYPERTENSION, 1997, 29 (02) :613-618
[8]   HYPERTENSION IN THE SPONTANEOUSLY HYPERTENSIVE RAT IS LINKED TO THE Y-CHROMOSOME [J].
ELY, DL ;
TURNER, ME .
HYPERTENSION, 1990, 16 (03) :277-281
[9]   THE HYPERTENSIVE Y-CHROMOSOME ELEVATES BLOOD-PRESSURE IN F11 NORMOTENSIVE RATS [J].
ELY, DL ;
DANESHVAR, H ;
TURNER, ME ;
JOHNSON, ML ;
SALISBURY, RL .
HYPERTENSION, 1993, 21 (06) :1071-1075
[10]   Transcription factor AP-1, and the role of Fra-2 [J].
Foletta, VC .
IMMUNOLOGY AND CELL BIOLOGY, 1996, 74 (02) :121-133