Ricin Activity Assay by Direct Analysis in Real Time Mass Spectrometry Release Detection of Adenine Release

被引：38

作者：

Bevilacqua, Vicky L. H. ^{[1
]}

Nilles, J. Michael ^{[2
]}

Rice, Jeffrey S. ^{[1
]}

Connell, Theresa R. ^{[2
]}

Schenning, Amanda M. ^{[2
]}

Reilly, Lisa M. ^{[1
]}

Durst, H. Dupont ^{[1
]}

机构：

[1] USA, Edgewood Chem Biol Ctr, Aberdeen Proving Ground, MD 21010 USA

[2] SAIC, Gunpowder Branch, Gunpowder, MD 21010 USA

来源：

ANALYTICAL CHEMISTRY | 2010年 / 82卷 / 03期

关键词：

RIBOSOME-INACTIVATING PROTEINS; N-GLYCOSIDASE ACTIVITY; A-CHAIN; EUKARYOTIC RIBOSOMES; RNA;

D O I：

10.1021/ac9025972

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

Biotoxin activity assays typically involve multistep sample preparation, multicomponent reactions, multistep analysis, or a combination thereof. We report a single-step, real-time ricin activity assay that requires little or no sample preparation and employs direct analysis in real time mass spectrometry. The release of adenine from the inhomogeneous substrate herring sperm DNA by ricin was determined to be 53 +/- 2 pmol adenine per picomole of ricin per hour. This procedure can be readily adapted to any enzyme for which a reactant or product of low molecular weight (up to similar to 600) can be identified.

引用

页码：798 / 800

页数：3

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