Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis

被引：113

作者：

Boutte, Yohann ^{[1
,2
]}

Frescatada-Rosa, Marcia ^{[1
]}

Men, Shuzhen ^{[1
,2
]}

Chow, Cheung-Ming ^{[3
]}

Ebine, Kazuo ^{[4
]}

Gustavsson, Anna ^{[1
]}

Johansson, Lenore ^{[5
]}

Ueda, Takashi ^{[4
]}

Moore, Ian ^{[3
]}

Juergens, Gerd ^{[6
]}

Grebe, Markus ^{[1
,2
]}

机构：

[1] Umea Univ, Umea Plant Sci Ctr, Dept Plant Physiol, SE-90187 Umea, Sweden

[2] Swedish Univ Agr Sci, Dept Forest Genet & Plant Physiol, UPSC, S-90183 Umea, Sweden

[3] Univ Oxford, Dept Plant Sci, Oxford, England

[4] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, Tokyo 113, Japan

[5] Umea Univ, Chem & Biol Ctr, Umea, Sweden

[6] Univ Tubingen, Dept Dev Genet, Ctr Plant Mol Biol ZMBP, Tubingen, Germany

来源：

EMBO JOURNAL | 2010年 / 29卷 / 03期

基金：

英国生物技术与生命科学研究理事会; 瑞典研究理事会;

关键词：

Arabidopsis; cytokinesis; endocytosis; KNOLLE syntaxin; sterols; PLASMA-MEMBRANE; LIPID RAFTS; SECRETORY TRAFFICKING; STEROL BIOSYNTHESIS; AUXIN TRANSPORT; PLATE FORMATION; H+-ATPASE; CHOLESTEROL; POLARITY; THALIANA;

D O I：

10.1038/emboj.2009.363

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytokinesis represents the final stage of eukaryotic cell division during which the cytoplasm becomes partitioned between daughter cells. The process differs to some extent between animal and plant cells, but proteins of the syntaxin family mediate membrane fusion in the plane of cell division in diverse organisms. How syntaxin localization is kept in check remains elusive. Here, we report that localization of the Arabidopsis KNOLLE syntaxin in the plane of cell division is maintained by sterol-dependent endocytosis involving a clathrin-and DYNAMIN-RELATED PROTEIN1A-dependent mechanism. On genetic or pharmacological interference with endocytosis, KNOLLE mislocalizes to lateral plasma membranes after cell-plate fusion. Fluorescence-loss-in-photo-bleaching and fluorescence-recovery-after-photo-bleaching experiments reveal lateral diffusion of GFP-KNOLLE from the plane of division to lateral membranes. In an endocytosis-defective sterol biosynthesis mutant displaying lateral KNOLLE diffusion, KNOLLE secretory trafficking remains unaffected. Thus, restriction of lateral diffusion by endocytosis may serve to maintain specificity of syntaxin localization during late cytokinesis. The EMBO Journal (2010) 29, 546-558. doi:10.1038/emboj.2009.363; Published online 3 December 2009

引用

页码：546 / 558

页数：13

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