A simplified laboratory validated assay for MGMT promoter hypermethylation analysis of glioma specimens from formalin-fixed paraffin-embedded tissue

被引：60

作者：

Cankovic, Milena

Mikkelsen, Tom

Rosenblum, Mark L.

Zarbo, Richard J.

机构：

[1] Henry Ford Hosp, Dept Pathol, Detroit, MI 48202 USA

[2] Henry Ford Hosp, Dept Neurosurg, Detroit, MI USA

来源：

LABORATORY INVESTIGATION | 2007年 / 87卷 / 04期

关键词：

formalin-fixed tissue; glioblastoma multiforme; methylation-specific PCR; MGMT; promoter hypermethylation; Temozolomide;

D O I：

10.1038/labinvest.3700520

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Glioma, and in particular high-grade astrocytoma termed glioblastoma multiforme (GBM), is the most common primary tumor of the brain. Epigenetic silencing of the MGMT (O-6-methylguanine-DNA Methyl transferase) DNA repair gene by promoter methylation compromises DNA repair and has been associated with longer survival in patients with GBM who receive alkylating agents. The methylation status of the MGMT promoter is determined by methylation-specific polymerase chain reaction analysis (MSP). This protocol is often challenging with GBM specimens, because of extensive necrosis and scarcity of malignant cells. The objective of this study was to develop a reliable, clinically validated assay for detection of epigenetic silencing of the MGMT gene using formalin-fixed, paraffin-embedded brain tumor resections and methylation-specific PCR.

引用

页码：392 / 397

页数：6

共 28 条

[1]

Andersson U, 2004, CLIN NEUROPATHOL, V23, P21

[2] DNA hypermethylation in tumorigenesis - epigenetics joins genetics [J].