Quantitative Live Single-cell Mass Spectrometry with Spatial Evaluation by Three-Dimensional Holographic and Tomographic Laser Microscopy

被引：35

作者：

Ali, Ahmed ^{[1
,2
]}

Abouleila, Yasmine ^{[1
,2
]}

Amer, Sara ^{[1
,2
]}

Furushima, Rie ^{[1
]}

Emara, Samy ^{[2
]}

Equis, Sebastien ^{[3
]}

Cotte, Yann ^{[3
]}

Masujima, Tsutomu ^{[1
]}

机构：

[1] RIKEN, Quantitat Biol Ctr QBiC, 6-2-3 Furuedai, Suita, Osaka 5650874, Japan

[2] Misr Int Univ, Fac Pharm, Km28 Ismailia Rd, Cairo, Egypt

[3] Nanol SA, Chemin Dent Oche 1a, CH-1024 Lausanne, Switzerland

来源：

ANALYTICAL SCIENCES | 2016年 / 32卷 / 02期

基金：

日本科学技术振兴机构;

关键词：

Quantitative live single-cell mass spectrometry; three-dimensional imaging; holographic and tomographic microscopy; quantitative single-cell analysis; HepG2; cell; LIGHT-MICROSCOPY; IMAGE-ANALYSIS;

D O I：

10.2116/analsci.32.125

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The locations and volumes of the contents of a single HepG2 cell were visualized under three-dimensional (3D) holographic and tomographic (HT) laser microscopy, colored by refractive index, not staining. After trapping the specific area of a target cell in a nanospray tip, quantification was performed by live single-cell mass spectrometry. Comparison of the HepG2 cells' before and after 3D-HT images allowed the inference of the precise volume and original location of the trapped cell contents. The total amount of a trapped molecule was estimated. The images also revealed morphological changes in the cell structure caused by the manipulation.

引用

页码：125 / 127

页数：3

共 10 条

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