A case for "StopGo'': Reprogramming translation to augment codon meaning of GGN by promoting unconventional termination (Stop) after addition of glycine and then allowing continued translation (Go)

被引：95

作者：

Atkins, John F. ^{[1
]}

Wills, Norma M.

Loughran, Gary

Wu, Chih-Yu

Parsawar, Krishna

Ryan, Martin D.

Wang, Chung-Hsiung

Nelson, Chad C.

机构：

[1] Univ Utah, Dept Human Genet, Salt Lake City, UT 84112 USA

[2] Natl Univ Ireland Univ Coll Cork, Biosci Inst, Cork, Ireland

[3] Natl Taiwan Univ, Dept Entomol, Taipei 10764, Taiwan

[4] Univ Utah, Mass Spectrometry & Proteom Core Facil, Salt Lake City, UT 84112 USA

[5] Univ St Andrews, Ctr Biomol Sci, St Andrews KY16 9ST, Fife, Scotland

来源：

RNA | 2007年 / 13卷 / 06期

基金：

英国惠康基金;

关键词：

codon redefinition; recoding; ribosome; picorna; 2A; StopGo;

D O I：

10.1261/rna.487907

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When a eukaryotic mRNA sequence specifying an amino acid motif known as 2A is directly followed by a proline codon, two nonoverlapping proteins are synthesized. From earlier work, the second protein is known to start with this proline codon and is not created by proteolysis. Here we identify the C-terminal amino acid of an upstream 2A-encoded product from Perina nuda picorna-like virus that is glycine specified by the last codon of the 2A-encoding sequence. This is an example of recoding where 2A promotes unconventional termination after decoding of the glycine codon and continued translation beginning with the 39 adjacent proline codon.

引用

页码：803 / 810

页数：8

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