Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion

被引：71

作者：

Fang, Ji-Kang

Prabu, Subbuswamy K.

Sepuri, Naresh B.

Raza, Haider

Anandatheerthavarada, Hindupur K.

Galati, Domenico

Spear, Joseph

Avadhani, Narayan G.

机构：

[1] Univ Penn, Biochem Lab, Dept Anim Biol, Sch Vet Med, Philadelphia, PA 19104 USA

[2] Univ Penn, Physiol Lab, Dept Anim Biol, Sch Vet Med, Philadelphia, PA 19104 USA

来源：

FEBS LETTERS | 2007年 / 581卷 / 07期

关键词：

cytochrome c oxidase; subunit phosphorylation; myocardial ischemia/reperfusion; protein kinase A; nano-LC-MS/MS analysis;

D O I：

10.1016/j.febslet.2007.02.042

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC-MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1302 / 1310

页数：9

共 24 条

[1] 3,5-diiodothyronine binds to subunit Va of cytochrome-c oxidase and abolishes the allosteric inhibition of respiration by ATP [J].