Validated MALDI-TOF/TOF mass spectra for protein standards

被引：57

作者：

Falkner, Jayson A. ^{[1
]}

Kachman, Maureen ^{[1
]}

Veine, Donna M. ^{[1
]}

Walker, Angela ^{[1
]}

Strahler, John R. ^{[1
]}

Andrews, Philip C. ^{[1
]}

机构：

[1] Univ Michigan, Dept Biol Chem, Michigan Proteome Consortium, Nat Resource Proteom & Pathway, Ann Arbor, MI 48109 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2007年 / 18卷 / 05期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/j.jasms.2007.01.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A current focus of proteomics research is the establishment of acceptable confidence measures in the assignment of protein identifications in an unknown sample. Development of new algorithmic approaches would greatly benefit from a standard reference set of spectra for known proteins for the purpose of testing and training. Here we describe an openly available library of mass spectra generated on an ABI 4700 MALDI TOF/TOF from 246 known, individually purified and trypsin-digested protein samples. The initial full release of the Aurum Dataset includes gel images, peak lists, spectra, search result files, decoy database analysis files, FASTA file of protein sequences, manual curation, and summary pages describing protein coverage and peptides matched by MS/MS followed by decoy database analysis using Mascot, Sequest, and X!Tandem. The data are publicly available for use at ProteomeCommons.org.

引用

页码：850 / 855

页数：6

共 23 条

[1] Role of accurate mass measurement (±10 ppm) in protein identification strategies employing MS or MS MS and database searching [J].