Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci

被引:236
作者
Poyart, Claire
Tazi, Asmaa
Reglier-Poupet, Helene
Billoet, Annick
Tavares, Nicole
Raymond, Josette
Trieu-Cuot, Patrick
机构
[1] Grp Hosp Cochin St Vincent de Paul, Assistance Publ Hop Paris, Serv Bacteriol, F-75679 Paris, France
[2] Grp Hosp Cochin St Vincent de Paul, Assistance Publ Hop Paris, Ctr Natl Reference Streptocoques, F-75679 Paris, France
[3] Univ Paris 05, Fac Med, U567, Inst Cochin,INSERM,CNRS,UMR 810, F-75014 Paris, France
[4] Inst Pasteur, Unite Biol Bacteries Pathogenes Gram Positif, CNRS, URA 2172, F-75724 Paris, France
关键词
D O I
10.1128/JCM.00159-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We developed a simple, specific, and sensitive two-multiplex-PCR assay that enabled the detection of all known group B streptococcal (GBS) capsular polysaccharides. This test is well adapted for GBS capsular polysaccharide typing in large-scale epidemiological studies.
引用
收藏
页码:1985 / 1988
页数:4
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