Expansion of adult Schwann cells from mouse predegenerated peripheral nerves

被引:33
作者
Verdú, E
Rodríguez, FJ
Gudiño-Cabrera, G
Nieto-Sampedro, M
Navarro, X [1 ]
机构
[1] Univ Autonoma Barcelona, Dept Cell Biol Physiol & Immunol, Neuroplast Grp, E-08193 Bellaterra, Spain
[2] CSIC, Inst Cajal, Neural Plast Grp, E-28002 Madrid, Spain
关键词
Schwann cell; defined medium; conditioned medium; sciatic nerve; nerve injury;
D O I
10.1016/S0165-0270(00)00221-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We present an effective technique for culture and expansion of Schwann cells (SC) from adult peripheral nerves. Cultures from adult mouse sciatic nerves (one to six nerves per culture) in defined medium showed markedly higher purity and density of SC when the nerve was predegenerated in vivo for 7 days than when it was harvested fresh. SC from degenerated nerves were then cultured in defined media conditioned by primary cultures of adult SC. The best results were obtained with a conditioned medium supplemented with 1% fetal calf serum. In these conditions the purity of SC was about 90% and the density about 190 cell/mm(2) by 7-10 days in vitro. These findings indicate that adult SC can be expanded from small preinjured nerve fragments in a short time period to provide a source of SC for autologous cellular transplants. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:111 / 117
页数:7
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