Application of atomic force microscopy to the study of micromechanical properties of biological materials

被引:75
作者
Bowen, WR [1 ]
Lovitt, RW [1 ]
Wright, CJ [1 ]
机构
[1] Univ Wales, Dept Chem & Biol Proc Engn, Ctr Complex Fluids Proc, Swansea SA2 8PP, W Glam, Wales
基金
英国生物技术与生命科学研究理事会;
关键词
adhesion; AFM; force measurement; nanoindentation; nanomanipulation;
D O I
10.1023/A:1005604028444
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Atomic force microscopy (AFM) has been used to study the micromechanical properties of biological systems. Its unique ability to function both as an imaging device and force sensor with nanometer resolution in both gaseous and liquid environments has meant that AFM has provided unique insights into the mechanical behaviour of tissues, cells and single molecules. As a surface scanning device, AFM can map properties such as adhesion and the Young's modulus of surfaces. As a force sensor and nanoindentor AFM can directly measure properties such as the Young's modulus of surfaces or the binding forces of cells. As a stress-strain gauge AFM can study the stretching of single molecules or fibres and as a nanomanipulator it can dissect biological particles such as viruses or DNA strands. The present paper reviews key research that has demonstrated the versatility of AFM and how it can be exploited to study the micromechanical behaviour of biological materials.
引用
收藏
页码:893 / 903
页数:11
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