Expression of 25 hydroxyvitamin D3-1α-hydroxylase in human endometrial tissue

被引：26

作者：

Becker, Steffi ^{[1
]}

Cordes, Tim ^{[1
]}

Diesing, Dagmar ^{[1
]}

Diedrich, Klaus ^{[1
]}

Friedrich, Michael ^{[1
]}

机构：

[1] Univ Schleswig Holstein, Dept Obstet & Gynaecol, D-23562 Lubeck, Germany

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2007年 / 103卷 / 3-5期

关键词：

extrarenal 1,25-dihydroxyvitamin D-3; 1; alpha-OHase; alternative splicing; endometrial cancer;

D O I：

10.1016/j.jsbmb.2006.12.075

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1,25(OH)(2)D-3 (calcitriol) has been shown to play an important role in cell proliferation, differentiation and immune responsiveness. The enzyme responsible for calcitriol synthesis 25 hydroxyvitamin D-3-1 alpha-hydroxylase (1 alpha-OHase) has been reported in many human tissues. The aim of this study was to investigate the expression of 1 alpha-OHase in gynaecological tissues. Using a highly specific nested touchdown PCR we examined the expression of 1 alpha-OHase in normal and malignant endometrial tissue and in human endometrial Ishikawa cells. In addition, we analyzed the protein expression of 1 alpha-OHase by Western blot. The expression of 1 alpha-OHase in normal and malignant endometrial tissue and Ishikawa cells was detected and splice variants of the enzyme in Ishikawa cells were identified. These data suggest an alternative splicing of 1 alpha-OHase in malignant endometrial tissue and cells. We postulate that the expression of 1 alpha-OHase gene variants may contribute to the anti proliferative effects of calcitriol. In conclusion, the modulation of the 1 alpha-OHase opens up a new target for vitamin D-3 related therapies in endometrial cancer. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：771 / 775

页数：5

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