Opioid withdrawal activates MAP kinase in locus coeruleus neurons in morphine-dependent rats in vivo

被引:71
作者
Schulz, S [1 ]
Höllt, V [1 ]
机构
[1] Otto Von Guericke Univ, Dept Pharmacol & Toxicol, D-39120 Magdeburg, Germany
关键词
antibody; dependence; immunocytochemistry; MAPkinase; opioid receptor; opioids; withdrawal;
D O I
10.1046/j.1460-9568.1998.00103.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Opioid dependence is widely believed to result from neuroadaptations in specific brain regions. However, the precise molecular mechanisms underlying these adaptations are not yet clear, Our aim was to explore the role of mitogen-activated protein kinase (MAPK) in mu opioid receptor signalling in vivo. Using anti-phospho MAPK antibodies, activated MAPK was detected in cortical neurons (layers II/III), median eminence, amygdaloid and hypothalamic nuclei in untreated animals. Dense nuclear and cytoplasmic staining was observed resulting in full visualization of processes in these cells. Chronic, but not acute, administration of morphine greatly diminished this staining pattern while mu opioid receptor levels and levels of MAPkinase as detected with a phosphorylation state-independent antibody were unchanged, When opioid withdrawal was precipitated with naloxone a dramatic increase in MAPkinase phosphorylation was observed in somata and fibres of locus coeruleus, solitary tract and hypothalamic neurons. Thus, the differential activation state of MAPK could have important implications for understanding the mechanisms underlying opioid tolerance and dependence.
引用
收藏
页码:1196 / 1201
页数:6
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