A procedure for characterizing glucans synthesized by purified enzymes of cariogenic Streptococcus mutans

被引：13

作者：

Aires, C. P. ^{[2
]}

Koo, H. ^{[3
,4
]}

Sassaki, G. L. ^{[1
]}

Iacomini, M. ^{[1
]}

Cury, J. A. ^{[2
]}

机构：

[1] Univ Fed Parana, Dept Biochem & Mol Biol, BR-81531980 Curitiba, Parana, Brazil

[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, BR-13414903 Piracicaba, SP, Brazil

[3] Univ Rochester, Med Ctr, Eastman Dept Dent, Rochester, NY 14620 USA

[4] Univ Rochester, Med Ctr, Ctr Oral Biol, Rochester, NY 14620 USA

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2010年 / 46卷 / 05期

关键词：

Streptococcus mutans; alpha-glucans; Chemical structure; GENE; SUCROSE; STARCH;

D O I：

10.1016/j.ijbiomac.2010.01.018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Extracellular polysaccharide is a virulence factor of the most cariogenic bacteria, Streptococcus mutans. We describe a procedure for chemical analysis of this polysaccharide of S. mutans, using a well-known glucan synthesized by purified enzymes of cariogenic streptococci. Soluble and insoluble glucan from glucosyltransferase D and B respectively, were prepared and analyzed by nuclear magnetic resonance spectroscopy and methylation. Soluble alpha-glucan contained 60% of (1 -> 6)-linkages and 17% of branching while insoluble alpha-glucan was composed of 57% of (1 -> 3)- and 28% of (1 -> 6)-linkages with 8% branching. The described procedure could be important focusing future studies on in vivo biofilm. (C) 2010 Published by Elsevier B.V.

引用

页码：551 / 554

页数：4

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