The cell wall-associated glyceraldehyde-3-phosphate dehydrogenase of Candida albicans is also a fibronectin and laminin binding protein

被引:197
作者
Gozalbo, D
Gil-Navarro, I
Azorín, I
Renau-Piqueras, J
Martínez, JP
Gil, ML
机构
[1] Univ Valencia, Fac Farm, Dept Microbiol & Ecol, Burjassot 46100, Valencia, Spain
[2] Hosp La Fe, Ctr Invest, Secc Biol & Patol Celular, E-46009 Valencia, Spain
关键词
D O I
10.1128/IAI.66.5.2052-2059.1998
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
By immunoelectron microscopy with a polyclonal antibody against the cytosolic glycolytic enzyme glyceralde-hyde-3-phosphate dehydrogenase (GAPDH) from Candida albicans (anti-GAPDH PAb), the protein was clearly detected at the outer surface of the cell wall, particularly on blastoconidia, as well as in tie cytoplasm, Intact blastoconidia were able to adhere to fibronectin and laminin immobilized on microtiter plates, and this adhesion was markedly reduced by both the anti-GAPDH PAb and soluble GAPDH from Saccharomyces cerevisiae. In addition, semiquantitative flow cytometry analysis with the anti-GAPDH PAb showed a decrease in antibody binding to cells in the presence of soluble fibronectin and laminin, Purified cytosolic C. albicans GAPDH was found to bind to fibronectin and laminin in a ligand Western blot assay. These observations suggest that the cell wall-associated form of the GAPDH in C. albicans could be involved in mediating adhesion of fungal cells to fibronectin and laminin, thus contributing to the attachment of the microorganism to host tissues and to the dissemination of Candida infection.
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页码:2052 / 2059
页数:8
相关论文
共 56 条
[1]   3-Phosphoglycerate kinase: A glycolytic enzyme protein present in the cell wall of Candida albicans [J].
Alloush, HM ;
LopezRibot, JL ;
Masten, BJ ;
Chaffin, WL .
MICROBIOLOGY-SGM, 1997, 143 :321-330
[2]   Identification of a glucan-associated enolase as a main cell wall protein of Candida albicans and an indirect target of lipopeptide antimycotics [J].
Angiolella, L ;
Facchin, M ;
Stringaro, A ;
Maras, B ;
Simonetti, N ;
Cassone, A .
JOURNAL OF INFECTIOUS DISEASES, 1996, 173 (03) :684-690
[3]  
Bodey G. P, 1993, CANDIDIASIS PATHOGEN
[4]   LAMININ RECEPTORS ON CANDIDA-ALBICANS GERM TUBES [J].
BOUCHARA, JP ;
TRONCHIN, G ;
ANNAIX, V ;
ROBERT, R ;
SENET, JM .
INFECTION AND IMMUNITY, 1990, 58 (01) :48-54
[5]  
Calderone R A, 1993, Arch Med Res, V24, P275
[6]  
CALDERONE RA, 1991, MICROBIOL REV, V55, P1
[7]   CHARACTERIZATION OF CELL-WALL PROTEINS FROM YEAST AND MYCELIAL CELLS OF CANDIDA-ALBICANS BY LABELING WITH BIOTIN - COMPARISON WITH OTHER TECHNIQUES [J].
CASANOVA, M ;
LOPEZRIBOT, JL ;
MARTINEZ, JP ;
SENTANDREU, R .
INFECTION AND IMMUNITY, 1992, 60 (11) :4898-4906
[8]   IDENTIFICATION OF A 58-KILODALTON CELL-SURFACE FIBRINOGEN-BINDING MANNOPROTEIN FROM CANDIDA-ALBICANS [J].
CASANOVA, M ;
LOPEZRIBOT, JL ;
MONTEAGUDO, C ;
LLOMBARTBOSCH, A ;
SENTANDREU, R ;
MARTINEZ, JP .
INFECTION AND IMMUNITY, 1992, 60 (10) :4221-4229
[9]   IDENTIFICATION OF WALL-SPECIFIC ANTIGENS SYNTHESIZED DURING GERM TUBE FORMATION BY CANDIDA-ALBICANS [J].
CASANOVA, M ;
GIL, ML ;
CARDENOSO, L ;
MARTINEZ, JP ;
SENTANDREU, R .
INFECTION AND IMMUNITY, 1989, 57 (01) :262-271
[10]   Cell wall and secreted proteins of Candida albicans:: Identification, function, and expression [J].
Chaffin, WL ;
López-Ribot, JL ;
Casanova, M ;
Gozalbo, D ;
Martinez, JP .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (01) :130-+