Primary structure and strand specificity of BstF5I-1 DNA methyltransferase which recognizes 5'-GGATG-3'

被引：10

作者：

Degtyarev, SK

Netesova, NA

Abdurashitov, MA

Shevchenko, AV

机构：

[1] SIBENZYME,NOVOSIBIRSK 63090 90,RUSSIA

[2] STATE RES CTR VIROL & BIOTECHNOL VECTOR,RES INST MOL BIOL,KOLTSOVO 633159,NOVOSIBIRSK,RUSSIA

来源：

GENE | 1997年 / 187卷 / 02期

关键词：

Bacillus stearothermophilus; class-IIS restriction-modification system; conserved motifs; recombinant DNA; RESTRICTION-MODIFICATION SYSTEM; SEQUENCE; CLONING; GENE;

D O I：

10.1016/S0378-1119(96)00752-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The gene for BstFSI-1 DNA-methyltransferase (MTase) from Bacillus stearothermophilus F5 (a FokI isoschizomer, recognizing 5'-GGATG-3') was cloned and its nucleotide (nt) sequence was determined. Analysis of deduced amino acid (aa) sequence shows that M . BstFSI-1 belongs to D-21 class of MTases and has a little homology with M . FokI. M . BstFSI-1 modifies only the upper strand of recognition sequence (5'-GGATG-3').

引用

页码：217 / 219

页数：3

共 7 条

[1] BstF5I, an unusual isoschizomer of FokI [J].