Microfluidic patterning of cells in extracellular matrix biopolymers: Effects of channel size, cell type, and matrix composition on pattern integrity

被引:103
作者
Tan, W
Desai, TA
机构
[1] Univ Illinois, Dept Bioengn, Sci & Engn Off, Chicago, IL 60607 USA
[2] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA
来源
TISSUE ENGINEERING | 2003年 / 9卷 / 02期
关键词
D O I
10.1089/107632703764664729
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The organization of cells within an extracellular matrix is critical to promote appropriate cellular interactions and tissue function in vivo. The ability to design and create biologically relevant cellular arrangements via microfluidic patterning on surfaces provides new capabilities for tissue engineering and biomimetics. The purpose of this article is to describe techniques using microfluidic patterning of three-dimensional biopolymer matrices to improve cellular pattern integrity and to provide microscale control over cellular microenvironments. Results demonstrated that the incorporation of extracellular matrix biopolymers in cell microfluidic patterning results in a more stable pattern of adherent human endothelial cells than patterning without matrix components after several days in vitro. This may be important for carrying out long-term biological experiments and tissue engineering in vitro. Moreover, chemical components in the patterned biopolymer matrices, such as collagen, chitosan, and fibronectin, influenced the ability of the matrices to control cell migration and pattern stability over time. Thus, microfluidic patterning of cells in extracellular matrix biopolymers was shown to be useful in patterning multiple cell types in well-defined three-dimensional geometries.
引用
收藏
页码:255 / 267
页数:13
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