A live bioprobe for studying diatom-surface interactions

被引：48

作者：

Arce, FT

Avci, R

Beech, IB

Cooksey, KE

Wigglesworth-Cooksey, B

机构：

[1] Montana State Univ, Dept Phys, MSU Phys, Bozeman, MT 59717 USA

[2] Univ Portsmouth, Sch Pharm & Biomed Sci, Portsmouth, Hants, England

[3] Montana State Univ, Dept Microbiol, Bozeman, MT 59717 USA

来源：

BIOPHYSICAL JOURNAL | 2004年 / 87卷 / 06期

关键词：

D O I：

10.1529/biophysj.104.043307

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Atomic force microscopy has been employed to compare the adhesion of Navicula species I diatoms to surfaces of a hydrophobic elastomer, Intersleek, and a hydrophilic mineral, mica. This was accomplished using tipless atomic force microscopy cantilevers functionalized with live diatom cells. Both surfaces were tested with the same diatom bioprobe. Force versus distance curves generated during these experiments revealed comparable cell adhesion strengths on Intersleek and mica, indicating that Navicula diatoms secrete extracellular polymeric substances with hydrophobic and hydrophilic properties. A statistical analysis of force curves was carried out and the average values of works of detachment of a diatom from Intersleek and mica surfaces were determined.

引用

收藏

页码：4284 / 4297

页数：14

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