iTRAQ compatibility of peptide immobilized pH gradient isoelectric focusing

被引：40

作者：

Lengqvist, Johan

Uhlen, Kristina

Lehtio, Janne

机构：

[1] Karolinska Univ Hosp, Karolinska Inst, Karolinska Biom Ctr, Stockholm, Sweden

[2] GE Healthcare, Uppsala, Sweden

来源：

PROTEOMICS | 2007年 / 7卷 / 11期

关键词：

multiplexing; peptide isoelectric focusing; relative quantitation;

D O I：

10.1002/pmic.200600782

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Immobilized pH gradient isoelectric focusing (IPG-IEF) has. emerged as a highly promising alternative to strong-cation exchange fractionation as the first dimension in shot-gun proteomics. Herein is shown the compatibility of this method with iTRAQ isotope labeling for relative quantitation and validation of sequence matches from database searching.

引用

收藏

页码：1746 / 1752

页数：7

相关论文

共 16 条

[1] THE FOCUSING POSITIONS OF POLYPEPTIDES IN IMMOBILIZED PH GRADIENTS CAN BE PREDICTED FROM THEIR AMINO-ACID-SEQUENCES [J].

BJELLQVIST, B ;

HUGHES, GJ ;

PASQUALI, C ;

PAQUET, N ;

RAVIER, F ;

SANCHEZ, JC ;

FRUTIGER, S ;

HOCHSTRASSER, D .

ELECTROPHORESIS, 1993, 14 (10) :1023-1031

[2]

Cargile Benjamin J, 2005, J Biomol Tech, V16, P181

[3] Gel based isoelectric focusing of peptides and the utility of isoelectric point in protein identification [J].

Cargile, BJ ;

Bundy, JL ;

Freeman, TW ;

Stephenson, JL .

JOURNAL OF PROTEOME RESEARCH, 2004, 3 (01) :112-119

[4] An alternative to tandem mass spectrometry: Isoelectric point and accurate mass for the identification of peptides [J].

Cargile, BJ ;

Stephenson, JL .

ANALYTICAL CHEMISTRY, 2004, 76 (02) :267-275

[5] Potential for false positive identifications from large databases through tandem mass spectrometry [J].

Cargile, BJ ;

Bundy, JL ;

Stephenson, JL .

JOURNAL OF PROTEOME RESEARCH, 2004, 3 (05) :1082-1085

[6] Immobilized pH gradients as a first dimension in shotgun proteomics and analysis of the accuracy of pI predictability of peptides [J].

Cargile, BJ ;

Talley, DL ;

Stephenson, JL .

ELECTROPHORESIS, 2004, 25 (06) :936-945

[7] A comparison of immobilized pH gradient isoelectric focusing and strong-cation-exchange chromatography as a first dimension in shotgun proteomics [J].

Essader, AS ;

Cargile, BJ ;

Bundy, JL ;

Stephenson, JL .

PROTEOMICS, 2005, 5 (01) :24-34

[8] Added value for tandem mass spectrometry shotgun proteomics data validation through isoelectric focusing of peptides [J].

Heller, M ;

Ye, ML ;

Michel, PE ;

Morier, P ;

Stalder, D ;

Jünger, MA ;

Aebersold, R ;

Reymond, FR ;

Rossier, JS .

JOURNAL OF PROTEOME RESEARCH, 2005, 4 (06) :2273-2282

[9] Two-stage Off-Gel™ isoelectric focusing:: Protein followed by peptide fractionation and application to proteome analysis of human plasma [J].

Heller, M ;

Michel, PE ;

Morier, P ;

Crettaz, D ;

Wenz, C ;

Tissot, JD ;

Reymond, F ;

Rossier, JS .

ELECTROPHORESIS, 2005, 26 (06) :1174-1188

[10] In-gel isoelectric focusing of peptides as a tool for improved protein identification [J].

Krijgsveld, Jeroen ;

Gauci, Sharon ;

Dormeyer, Wilma ;

Heck, Albert J. R. .

JOURNAL OF PROTEOME RESEARCH, 2006, 5 (07) :1721-1730